摘要
为研究载脂蛋白（ａｐｏ）Ｅ基因与脑血栓疾病间的关系，应用聚合酶链－限制性片段长度多态性（ＰＣＲ－ＲＦＬＰ）方法先扩增出ａｐｏＥ基因第４外显子内的２２７ｂｐ片段，继以限制性内切酶ＣｆｏＩ酶解消化，８％聚丙烯酰胺凝胶电泳，电后银染显带判定基因型，并计算等位基因频率。本实验共检测了正常汉族１１３例及３７例汉族脑血栓患者ａｐｏＥ基因型及等位基因频率。结果表明脑血栓患者ａｐｏＥε４／３基因型和ε４等位基因频率显著高于正常对照（Ｐ＜００１和Ｐ＜００５）。说明该方法快速、准确、分辨率高，ａｐｏＥ等位基因多态与脑血栓疾病间存在相关。
The relationship between apolipoprotein E(apoE) gene polymorphism and cerebral thrombosis was studied.The polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was used to amply 227 bp DNA fragment in the fourth exon of apoE gene and to digest them with restriction enzyme CfoI,then on 8% polyacrylamid gel electrophoresis for the isolation of the enzymic products,subsequently genotype was determined by silver staining and allele frequencies were counted in 113 normal Han nationality people and 37 cases with cerebral thrombosis.The results showed that the heterozygous genotyping ε 4/3 and the frequencies of ε 4 allele were significantly higher in the patients with cerebral thrombosis than that in age matched controls ( P <0 01 and P <0 05).Advantages of the method are rapid,exact and higher resolution ratio.It suggests that the ε 4 allele increased the risk for cerebral thrombosis.
出处
《中国医师杂志》
CAS
1999年第7期22-24,共3页
Journal of Chinese Physician
