摘要
应用ＰＣＲ－ＲＦＬＰ技术研究我国汉族人群ａｐｏＥ基因型与等位基因频率分布特征，并分析ａｐｏＥ基因多态性对脂质代谢的影响。发现我国汉族人群ａｐｏＥε３等位基因频率（８３．２％）明显高于欧美人群，而ε４频率（７．５％）显著低于欧美人群。ε４与血清ＴＣ、ＬＤＬ－Ｃ和ａｐｏＢ水平增加呈显著正相关。ａｐｏＥ等位基因变异对血清ＴＧ、ＨＤＬ－Ｃ、ａｐｏＡＩ及Ｌｐ（ａ）水平无明显影响。研究提示，ａｐｏＥ基因多态性影响血清脂质和脂蛋白水平，我国汉族人群ａｐｏＥ基因多态性特征可能与中国人群冠心病患病率明显低于欧美人群相关。
The apolipoprotein E (apoE) polymorphism in a Han Chinese population was analyzed with the method of polymerase chain reaction and restriction fragment length polymorphism (PCR RFLP). The effects of gene polymorphism of apoE on lipids, apolipoproteins and Lp(a) were also studied. The results showed that the distribution of apoE alleles (ε2,9.3%;ε3,83.2%;ε4,7.5%) was significantly different from those of the European and American population owing to a higher frequency of the ε3 allele but a lower frequency of the ε4 allele. We found significantly higher serum TG,LDL C,and apoB levels in individuals carrying ε4,but no differences were found in serum TG,HDL C,apoAI or Lp(a) by alleles. Data suggested the genetic polymorphism of apoE obviously influenced serum lipid profiles and associated with the relatively low cardiovascular mortality rate in Han Chinese.
出处
《基础医学与临床》
CSCD
1998年第1期48-52,共5页
Basic and Clinical Medicine
关键词
载脂蛋白E
基因型
聚合酶链反应
冠心病
血脂
apolipoprotein E genotype polymerase chain reaction coronary heart disease
