摘要
本试验以日照红茶为材料,在单因素试验基础上,采用Box-Behnken响应面设计法对茶多酚超声波辅助提取清除DPPH的工艺进行优化,建立了乙醇体积分数、超声时间、超声温度、乙酸乙酯萃取静置时间4因素与DPPH清除率之间的回归优化模型,确定了最佳提取工艺为:乙醇体积分数86.54%,超声时间69.61min,超声温度60℃,静置萃取时间82.26min,在优化后的最佳工艺参数下,红茶茶多酚对DPPH的清除率最高可达74.84%,研究表明红茶茶多酚具有较强的清除DPPH的能力。
the experiment with black tea as the materials, in a single factor experiment based on the Box-Behnken response surface design method of tea polyphenols utrasonic-assisted extraction process optimization, established the ethanol volume fraction, ultrasonic time, ultrasonic temperature, ethyl acetate extract stalling time four factors and the return of the clearance between DPPH optimization model, Determine the best extraction process for： ethanol volume fraction of 86.54%, ultrasonic time 69.61 rain, ultrasonic temperature 60 ℃, quiet place extraction time 82.26 rain, after optimization in the best technical parameters, black tea in the tea polyphenol DPPH extract on the largest clearance, can reach 74.84%. Studies show that black tea polyphenol extract has strong ability of clear DPPH.
出处
《茶叶通讯》
2013年第1期3-6,14,共5页
Journal of Tea Communication
