摘要
目的优选金荞麦中表儿茶素的提取工艺。方法以微波功率、乙醇浓度、料液比、提取时间为自变量,表儿茶素提取率为因变量,通过对自变量各水平的多元线性回归及二项式拟合,用星点设计-效应面法选取最佳工艺,并进行预测分析。结果最佳工艺条件为微波功率211.89 W,乙醇浓度55.11%,料液比1∶11.43,提取时间6.16 min,金荞麦中表儿茶素提取率的最大估计值为2.041 4%。实验结果与模型预测值相符。结论该方法简便合理,稳定,可预测性较优,可作为金荞麦的提取工艺。
Objective To optimize the extraction technology of epicatechin from Fagopyrum dibotrys （ D. Don） Hara. Methods Central composite design and response surface methodology were adopted to optimize the process of extraction. The independent variables were microwave power, ethanol concentration, sample/solvent ratio and extraction time. The dependent variable was extraction rate of epicatechin which was used to estimate the relationship between independent and dependent variables. The prediction was evaluated by comparing the observed and predicted values. Results The optimum technological parameters were as follows ： microwave power 211.89 W, ethanol concentration 55.11% , ratio of sample/solvent 1： 11.43, and extraction time 6.16 min. The highest extraction rate of epicatechin was 2.041 4% , which coincided with the model predicted values. Conclusion The extraction technology is simple,reliable and highly predictive.
出处
《医药导报》
CAS
北大核心
2013年第2期226-229,共4页
Herald of Medicine
基金
黑龙江省教育厅面上项目(12511370)
关键词
金荞麦
表儿茶素
提取工艺
星点设计
效应面法
Fagopyrum dibotrys （D. Don） Hara
Epicatechin
Optimization of extracting technology
Centralcomposite design
Response surface methodology
