摘要
目的：建立可测定金荞麦饮片中表儿茶索含量的高效液相色谱-电化学检测法。方法：采用Diamonsil C18（4．6mm×250mm，5μm）柱，流动相甲醇-0．1mol·L^-1磷酸盐缓冲液（1：3，pH2．5），流速1．0mL·min^-1，柱温35℃，参比电极ISAAC（in-situ silver／silver chloride），工作电极玻碳电极，检测电位＋600mV。结果：表儿茶素的线性范围为0．004875～1．56μg，r=0．9999；平均回收率为100．7％，RSD 1．9％（n=5）。9批不同市售来源的金养麦饮片中表儿茶素的含量存在差异。结论：该方法重复性好，可以用来测定金荞麦饮片中表儿茶素的含量。
Objective： To establish a quantitative method of determination of epieatechin in Rhizoma fagopyri dibotoryis by HPLC-electroebemical detection. Method： The sample was separated on a column of Diamonsil C18（4.6 min× 250 mm, 5 μm） which was eluted with methanol-0.1 mol·L^- 1phosphate buffer （1：3, pH 2.5）, the flow rate was 1.0 mL·min^-1, the column temperature was 35 ℃, the reference electrode was ISAAC（in-situ silver/silver chloride）, the work electrode was glassy carbon, the applied potential was ＋ 600 mV. Result： The linear range of epieatechin was 0.004 875-1.56 μg, r = 0.999 9. The average recovery was 100.7 % with RSD 1.9% （ n = 5）. The content of epieatechin was different in nine samples purchased. Conclusion： The method is good reproducible and can be used to determine epicatechin in Rhizoma fagopyri dibotoryis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第2期117-119,共3页
China Journal of Chinese Materia Medica
基金
北京市科技新星计划资助项目(H020821410190)
