摘要
目的:建立舒血宁注射液中5-羟甲基糠醛(5-HMF)的高效液相色谱(HPLC)测定方法。方法:采用Agi-lent Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱;甲醇-水(5∶95)为流动相,流速0.8 ml/min,检测波长284nm,柱温30℃。结果:5-HMF在0.099 80～49.902μg/ml范围内呈良好的线性关系(r=0.9999),平均回收率(n=9)为100.06%,RSD为0.45%。结论:该方法简便、准确、重复性好,可作为舒血宁注射液的质量控制方法。
Objective： To establish a method for the determination of the 5-hydroxymethyfurfural（5-HMF） in Shuxuening Injection by HPLC.Methods： Agilent Eclipse XDB-C18（250 mm×4.6 mm,5 μm） column was used with the mobile phase of methanol-water（5∶95） and the flow rate was 0.8 ml/min.The detection wavelength was 284 nm.Results： The linear range of 5-HMF was 0.099 80~49.902 μg/ml（r=0.9999）.The average recovery（n=9） was 100.06% with RSD=0.45%.Conclusion： This method was simple,accurate,reproducible,and can be used for the quality control of Shuxuening Injection.
出处
《天津药学》
2013年第1期7-9,共3页
Tianjin Pharmacy
