摘要
目的:研究以HPLC法测定小半夏汤中5-羟甲基糠醛含量的方法。方法:采用Lichrospher C18柱(4.6 mm×150 mm,5μm),以甲醇-水(5∶95)为流动相,体积流量1.0 mL·min-1,波长283 nm,柱温30℃。结果:5-羟甲基糠醛在0.304～2.736μg与峰面积呈现良好的线性关系,r=0.999 9;平均加样回收率(n=5)为96.18%,RSD为1.67%。结论:该法操作简便,结果可靠,重复性好,可作为研究小半夏汤中5-羟甲基糠醛含量的方法。
Objective： To establish an HPLC method for determination of 5-hydroxymethylfurfural in Xiaobanxia Tang.Method：A Lichrospher C18 column（4.6 mm × 150 mm,5 μm） was used with methanol-water（5 ∶ 95） as mobile phase.The flow rate was 1.0 mL.min-1,wavelength 283 nm,and the column temperature was at 30 ℃.Result：The linear range of 5-hydroxymethylfurfural was within 0.304-2.736 μg,r = 0.999 9,the average recovery was 96.18%（n = 5）.Conclusion：This method has good reproducibility.It can be used for quality control in production of Xiaobanxia Tang.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第13期51-52,55,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏省中医药管理局科技项目(LZ09027)
