摘要
采用高效液相色谱法,对镇江香醋中5-羟甲基糠醛进行了定量分析.样品中的HMF含量的测定条件为:以甲醇-水溶液(体积比为5∶95)为流动相,流速为1.0 mL/min,采用Venusil XBP-C18色谱柱分离,柱温为30℃,UV检测器的检测波长为284 nm.定量分析结果表明,镇江香醋中HMF的含量为8.01 mg/kg.高效液相色谱法分析镇江香醋中HMF含量的方法测定准确,稳定性高,HMF在0.014 3～0.085 8μg的进样范围内线性相关系数为R2=0.999 9,平均回收率98.66%,相对标准偏差为5.53%.
A method for determination of 5-hydroxymethylfurfural by high-performance liquid chromatography in Zhenjiang vinegar was developed.Samples were analyzed on a Venusil XBP-C18 column with oven temperature of 30 ℃,using a mixture of methanol and water（5∶95,v/v） as the mobile phase with the flow rate of 1.0 mL/min,and detected at 284 nm.Within the linear range of 0.014 3～0.085 8 μg,the correlation coefficient was 0.999 9.The method was accurate with the average recoveries rate and relative standard deviation of 98.66% and 5.53%,respectively.
出处
《北京工商大学学报（自然科学版）》
CAS
2012年第2期39-42,共4页
Journal of Beijing Technology and Business University:Natural Science Edition
基金
国家"十二五"科技支撑计划项目(2011BAD23B01)
关键词
镇江香醋
高效液相色谱
5-羟甲基糠醛
含量检测
Zhenjiang vinegar
high-performance liquid chromatographic
5-hydroxymethylfurfural
content determination
