摘要
以银杏提取物的酶水解液为实验材料,研究大孔吸附树脂分离纯化其中的银杏黄酮苷元的工艺。通过静态吸附-解吸和吸附动力学实验,从3种大孔吸附树脂中筛选出非极性YWD07a为最适合分离纯化银杏黄酮苷元的树脂。YWD07a树脂吸附分离银杏黄酮苷元的最佳工艺条件为:吸附:银杏黄酮苷元的浓度3.26 mg/mL,pH 5.0,流速2 BV/h;解吸:洗脱体积6BV,流速3.0 BV/h,乙醇浓度70%。在最优条件下得到的银杏黄酮苷元产品中银杏黄酮苷元含量由18.5%提高到80.5%,回收率为73.6%。
In this study, the separation effect of macroporous resin for the purification ot gmRgo tlavonol aglycone prepared by the enzymatic hydrolysis of the Immobilized B -glucosidase was developed. Based on the results of static adsorption and desorption, adsorption dynamic, YWDO7a resin was the best one for the separation and en- richment of flavonol aglycone. The optimal separation conditions were as follow： on adsorption, flavone aglycone concentration of sample solution, pH, flow rate was 3.3 mg/mL, 5. O, 2 bed volume （BV） per hour, respec- tively. ; on desorption, volume of elution solvent, flow rate, enthaol concentration was 6 BV, 3 BV/h, 70 % （v/v）, respectively. Under these conditions, the content of flavone aglycone increased from 18.5% to 80.5% in the crude oroduct, and the recovery of flavone aglycone reached 73.6 %.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2013年第1期40-45,共6页
Journal of Shandong Agricultural University：Natural Science Edition
关键词
银杏叶
分离纯化
大孔树脂
银杏黄酮苷元
Ginkgo leaf
separation and purification
macroporous resin
Ginkgo flavone aglycone
