摘要
目的:观察维生素E琥珀酸酯(VES)对H22荷瘤小鼠的抑瘤作用,并探讨其作用机制。方法:将40只移植了H22肝癌瘤株的昆明种小鼠随机分成4组:模型对照组(仅腹腔注射芝麻油);VES低、中、高浓度组(分别予以VES 50、100、200 mg.kg-1),每组连续用药12 d。比较各组肿瘤重量、肿瘤生长抑制率、肿瘤细胞周期的变化、血清中TNF-α及IL-2的表达。结果:50 mg.kg-1VES组肿瘤重量为(1.98±0.56)g,与模型对照组比较,无肿瘤生长抑制作用(P>0.05);100、200 mg.kg-1VES组肿瘤重量分别为(1.15±0.32)g、(0.90±0.19)g,肿瘤生长抑制率分别为46.76%、58.33%,与模型对照组比较,差异均有统计学意义(P<0.05),且表现为浓度依赖关系;VES各组均使G0/G1期细胞比例增加,S期细胞比例降低;VES 100、200 mg.kg-1可显著增加小鼠血清中TNF-α及IL-2的表达(均P<0.05)。结论:VES在体内对小鼠H22肿瘤细胞的生长有明显的抑制作用,其作用机制可能与阻滞细胞周期于G0/G1期、上调血清中TNF-α及IL-2表达有关。
Objective： To observe the antitumor effect of vitamin E succinate （VES） on H22 hepatoma- bearing mice and explore its mechanism. Methods： Forty mice embedded with H22 hepatoma were randomly divided into 4 groups： model control group（ which were injected sesame oil into intraperitoneal only） , VES low, medium and high concentrationgroups （which were given VES 50,100 and 200 mg · kg^-1 respectively ）. Each group received corresponding treatment for 12 days. Tumor weight, inhibition rate of tumor,growth, the cell cycle, expression of TNF-α and IL-2 in serum were compared among them. Results： Tumor weight of 50 mg · kg^-1 VES group was （1.98 ±0. 56） g, compared with model control group, there was no inhibitory effect （P 〉0. 05）. However,tumor weight of 100 and 200 mg ·kg^-1 VES groups were（ 1.15 ± 0.32）and （0. 90 ±0. 19） g and inhibition rates were 46.76% and 58.33%, respectively（ all P 〈0.05）. This effect was concentration dependencies. The percentage of G0/G1 phase cells increased and S phase cells decreased in each VES group. Expression of TNF-ot and IL-2 in serum increased in 100 and 200 mg · kg^-1 VES groups all（ P 〈 0.05 ）. Conclusion： VES can significantly inhibit growth of H22 hepatoma in vivo, the mechanism may be realized by cell cycle blockage at the G0/GI phase and increase of TNF-α and IL-2 expression in serum.
出处
《现代医学》
2013年第3期177-180,共4页
Modern Medical Journal
