摘要
目的:研究中药苦参碱(Matrine)的抗肿瘤作用及其免疫学机制。方法:MTT比色法检测肿瘤细胞的生长抑制率;流式细胞术分析细胞周期的改变。建立荷瘤小鼠模型,观察小鼠肿瘤的生长情况,计算肿瘤生长抑制率;MTT法测定苦参碱对荷瘤小鼠PBMC体外增殖作用的影响;ELISA法检测苦参碱治疗后小鼠血清中IL-2和IL-12的活性。结果:苦参碱可显著抑制小鼠肿瘤细胞的体外分裂和增殖,且抑制作用呈浓度和时间依赖性,使G1期细胞增多,S期和G2/M期细胞减少,细胞增殖指数降低。苦参碱对小鼠实体瘤生长具有显著抑制作用,抑瘤率高达60.7%以上;明显抑制小鼠静止PBMC的体外增殖作用;不能提高荷瘤小鼠血清中IL-2和IL-12的含量。结论:苦参碱具有较强的抗肿瘤作用,体内体外却表现出一定的免疫抑制作用,提高机体的免疫功能不是苦参碱发挥抗癌活性的主要机制。
Objective：To explore the inhibitory effects of matrine on tumor growth and the possible mechanisms related to immunological functions. Methods：The tumor cell growth repression rate was measured by MTT colorimetry. Flow cytometry was used to analyze the cell cycle of H22 cells. The H22 BALB/c xenograft tumor models were established. The anti-tumor effect of matrine was observed and tumor growth inhibitory rate（IR） was calculated. The effect of matrine on the proliferation of peripheral blood mononuclear eell（PBMC） separated from H22 tumor-bearing BALB/c was determined by MTT assay in vitro; the content of serum IL-2 and IL-12 were examined by ELISA assay. Results： Matrine inhibited proliferation of the H22 tumor cells in both time and concentration-dependent manners, with increased the cell numbers at G1 phase and decreased numbers at S and G/M phase showing the possible mechanism for lowering cell proliferation rate. The tumor inhibition rate of matrien came up to 60. 7% in tumor-bearing mice. Matrine significantly suppressed the proliferative activity of PBMC from tumor-beating mice. Furthermore, martine could not increase the contents of murine serum IL-2 and IL-12. Conclusion： Matrine could inhibit tumor cell growth directly but not exert the anti-tumor efficiency through reinforcing the immune function.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2007年第5期434-437,共4页
Chinese Journal of Immunology
基金
国家自然科学基金资助项目(基金号:30171150)
关键词
苦参碱
肿瘤
免疫调节
Matrine
Tumor
Immunoloregulation
