摘要
为了评价杜仲叶分离纯化产物抗疲劳功效,测定经大孔吸附树脂分离纯化后两种分离纯化产物中多酚含量,并采用动物实验评价纯化产物对小鼠负重游泳时间、血乳酸(LA)、血清尿素氮(SUN)、肝糖原和肌糖原以及乳酸脱氢酶(LDH)的影响,比较各纯化产物的抗疲劳功效。结果表明:多酚含量最高的固体纯化产物为EU1Ⅰ(20%乙醇洗脱液纯化产物),多酚含量最低的固体纯化产物为EU1Ⅱ(60%乙醇洗脱液纯化产物);EU1Ⅰ的抗疲劳功效强于EU1Ⅱ;多酚类物质可能为杜仲叶树脂分离纯化产物表现抗疲劳功效的主要功效成分。
In order to explore the anti-fatigue effect of Eucommia ulmoides leaves,the polyphenols in the extract from Eucommia ulmoides leaves was separated and purified by resin.The anti-fatigue effect of the extract was investigated by determining weight-loaded swimming time,blood lactic acid(LA),serum urea nitrogen(SUN),hepatic glycogen,muscle glycogen and lactate dehydrogenase(LDH)in mice.The results showed that the content of polyphenols in EU1Ⅰ(20%ethanol eluted product)was higher than that in EU1Ⅱ(60%ethanol eluted product),and the anti-fatigue effect of EU1Ⅰwas better than EU1Ⅱ,suggesting that polyphenols might be major components of anti-fatigue capacity in Eucommia ulmoides leaves.
作者
刘迪
尚华
宋晓宇
LIU Di;SHANG Hua;SONG Xiao-yu(College of Chemical Engineering and Textile,Shaanxi Polytechnic Institute,Xianyang 721000,China;Shaanxi Halsheng Fresh Fruit Juice Co.Ltd.,Qian County Branch,Xi’an 713300,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第5期251-254,共4页
Food Science
基金
陕西省教育厅专项科研计划项目(12JK0600)
陕西工业职业技术学院自选科研计划项目(ZK11-31)
关键词
杜仲叶
分离纯化产物
抗疲劳
多酚
Eucommia ulmoides leaves
extract
anti-fatigue
polyphenols
