摘要
目的:建立华蟾素注射液中总蟾毒配基类成分的含量测定方法。方法与结果:采用紫外分光光度法测定了华蟾素注射液中总蟾毒配基类成分的含量。测定结果为酯蟾毒配基在6.4～32μg线性良好,其回归方程为Y=0.005 66X+0.027 6(r=0.999 9)。5批华蟾素注射液中总蟾毒配基类成分的含量4.62～5.80 mg.L-1。结论:方法简便,重复性好,可以作为华蟾素注射液中总蟾毒配基类成分含量测定的质控方法之一。
Objective： To set up the determination of total bufadienolides content in cinobufotalin injection by UV. Method： A rapid and validated method was established for the determination of total bufadienolides in cinobufacini injection using ultraviolet spectrophotometry. Result： Total bufadienolides in cinobufacini injection were analyzed with acceptable linear regression relationship from 6.4 to 32 μg.The regression equation was Y=0.005 66X＋0.027 6（r=0.999 9）.Five batches of cinobufacini injection were included in the analysis.The content of total bufadienolides was from 4.62 to 5.80 mg·L-1. Conclusion： A simple method with good repeatability was established, which was benefit for the quality control of total bufadienolides in cinobufacini injection.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第6期87-89,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
重大新药创制项目(2009ZX09103-404)
中国中医科学院自主课题(Z02063)
关键词
华蟾素注射液
总蟾毒配基
含量测定
Cinobufotalin injection
total bufadienolides
determination
