摘要
目的研究脂多糖（LPS）及伴刀豆球蛋白A（ConA）诱导脾淋巴细胞增殖试验的两种方法用于免疫毒性评价的可行性。方法雄性Wistar大鼠40只，体重180～200g，随机分为对照组和2，5，10mg／kg环磷酰胺染毒组，每组10只大鼠，各剂量组每日灌胃染毒1次，连续28d，对照组给予生理盐水。染毒结束后24h，断头处死大鼠，取脾脏，制浓度为3×10^6个／ml的脾细胞悬液，用MTT方法进行LPS及ConA诱导脾淋巴细胞增殖试验。结果10mg／kg环磷酰胺染毒组大鼠的B淋巴细胞和T淋巴细胞的增殖能力（吸光度值，分别为0．032±0．037和0．028±0．050）低于对照组的B淋巴细胞和T淋巴细胞的增殖能力（分别为0．124±0．093和0．458±0．320），差异有统计学意义（P〈0．05）。结论LPS及ConA诱导脾淋巴细胞增殖试验的两种方法用于免疫毒性评价具有可行性。
Objective To show the feasibility of test procedures of lipopolysaccharide（LPS）-induced and concanavalin A（ConA）-induced rat splenocyte proliferation in assessment of immunotoxicity. Methods 40 male Wistar rats with body weight of 180-200 g were randomly and equally divided into control group and groups of 2, 5, and 10 mg/kg cyclophosphamide. The rats were exposed to cyclophosphamide by gavage once a day successively for 28 days. Normal saline was given to the control group. 24 hours after the ending of the exposure, the rats were sacrificed by decapitation and their spleens were excised and prepared into spleen single cell suspention of 3 × 10^6 cells/ml. LPS-induced and ConA-induced splenocyte proliferation was determined by MTT assay. Results LPS-induced and ConA-induced splenocyte proliferation in the group of lOmg/kg cyclophosphamide （absorbance： 0. 032 ± 0. 037 and 0. 028 ± 0. 050, respectively） were significantly lower than those of the control group（0. 124±0. 093 and 0. 458±0. 320, respectively）, P〈0.05. Conclusions The results showed that the test procedures of LPS-induced and ConA-induced splenocyte proliferation are feasible to be used in immunotoxicity assessment.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2007年第6期336-339,共4页
Industrial Health and Occupational Diseases
