摘要
核酶(Ribozyme)是具有催化RNA切割反应功能的RNA,它可以特异性地切割RN-A。最近,核酶的一级结构已经被证实,其与底物结合时所形成的二级结构如锤头状(Hammerhead)、发夹型(Hairpin)结构,亦得到初步证实。因此,能够设计并人工合成核酶,通过特异性破坏RNA,阻断其功能,从而有效地阻止病毒的复制和繁殖。这是近年来继反义RNA之后发现的又一个抑制基因表达的有力工具,开辟了分子生物学的一个新领域。
Ribozyme is a kind of RXA which can catalyze cleavage of the sequence-specific RNA. However, there have been no direct evidence for Ribozyme's spatial structure. We applied the technology of Scanning Tunnelling Microscopy ( STM ) to intend to get some direct information, about the spatial structure of the hammerhead-structure Ribozyme,which was artificial synthesized and designed to the transcript of HBsAg gene. The Ribozyme's molecular length and its greatest width was 286.8 A and 20.0 A. The Ribozyme molecules stretched out linearly on graphite surface and were not as expected to form a hairpin or other complicated structure.
出处
《病毒学报》
CAS
CSCD
北大核心
1991年第4期369-371,共3页
Chinese Journal of Virology
