摘要
利用组建的苜蓿尺蠖核型多角体病毒(AcNPV)非融合蛋白基因转移载体将乙型肝炎病毒表面抗原(HBsAg)基因成功地插入粉纹夜蛾(Tn)NPV中,HBsAg基因在感染了重组病毒的草地夜蛾(Spodontera frugiperda)离体细胞以及粉纹夜蛾和蓖麻蚕等虫体中获得了表达,免疫电镜下观察到典型的乙型肝炎病毒表面抗原22um颗粒。感染重组病毒的蓖麻蚕预蛹每克蛹重可产HBsAg蛋白1.6μg。表达产物经DEAE-纤维素层析得到的HBsAg粗提物可作临床检测用,再经抗体亲和柱层析可得到纯的HBsAg。
The gene coding for the hepatitis B virus surface antigen (HBsAg)under the control of Autographa californica nuclear polyhedrosis viruspolyhedrin promoter was successfully inserted into the genome of Tricho-plusia ni nuclear polyhedrosis virus. Infection of Spodoptera frugiperdacells with this recombinant virus produced significant amount of HBsAgprotein and secreted 22 nm particles containing the HBsAg. HBsAg gene expression was also obtained both in Trichoplusia ni larvae and in Philo-samia cynthia ricini prepupae when infected with the recombinant virus.The HBsAg proteins expressed by baculovirus vector systems are morpho-logical and antigenic properties identical to the 22 nm particles secretedby human cells.
出处
《生物工程学报》
CAS
CSCD
北大核心
1991年第1期37-46,共10页
Chinese Journal of Biotechnology
关键词
重组病毒
昆虫体系
HBsAg基因
Hepatitis B virus surface antigen
recombinant baculovirus
Trichoplusia ni
Philosamia cynthia ricini
Spodoptera frugiperda cells
