摘要
本文根据Ｈａｓｅｌｏｆｆ和Ｇｅｒｌａｃｈ发表的锤头状核酶结构，设计合成了针对ＨＢＶａｙｗ株Ｐ基因５＇－端２３６０位点（ＧＵＣ）的核酶ＲＣＰ，其作用底物为ＨＰＶａｙｗＰ基因的翻译起始区（２１４０－２４２２区段），运用基因克隆结合体外转录的方法，评价了ＲＣＰ的切割活性，结果表明，ＲＣＰ在体外成功地切割了长为３４０个核苷酸的靶ＲＮＡ分子，这一工作为我们进一步在细胞内评价ＲＣＰ抑制ＨＢＶ全基因组复制及其基因表达的研究创造了条件。
Based on the previous studies with the antisense RNA technique that the best inhibition effect on HBV gene expression carne from the 5'-end and non-coding region of P gene,we designed and synthesized a hammerhead ribozyme(RCP)which targeted at the 5'-end of the P gene at the 2360 site on the HBV ayw genome.Results showed that this ribozyme sucsessfully cleaved its substrate in vitro.The results provide a basis for further study for its blockign effect on HBV gene expression and replication in vivo.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1995年第3期187-189,共3页
Journal of Third Military Medical University
基金
全军"八.五"青年科学基金
