摘要
中药有效成分半抗原抗体的应用已经触及中药研究的各个领域。首先,可将半抗原单克隆抗体设计成ELISA试剂盒,定量分析生物样本或植物粗提取物中有效成分的含量,应用于中药质量控制和中药药代动力学的研究。其次,可将半抗原单克隆抗体与固相载体相偶联,做成免疫亲和柱,应用于复杂样品的前处理以及knock-out提取物的制备。最后,可将有效成分半抗原单克隆抗体单链可变区(scFv)的基因片段导入植物细胞内,基因重组后可得到有效成分高富集的新品种,实现药用植物的分子育种。
Hapten antibodies are active components of traditional Chinese medicines, have been widely applied in all of study fields of traditional Chinese medicine. First, hapten monoclonal antibodies could be designed into ELISA kits for quantitative analysis on the content of effective components in plant crude extracts or biological samples, which be applied for quality control and studies on pharmacokenetics of traditional Chinese medicines. Second, hapten monoclonal antibodies could be coupled with solid-phase carriers to generate immunoaffinity chromatography column, which could be used for knock-out extract preparation or pre-treatment of complicated sampless. Finally, a single-chain variable fragment antibody （scFV） gene segment of effective components of hapten monoclonal anti- bodies could be transformed into relative plant ceils to gain new varieties with high-enrichment effective components, and thus achieve the molecular breeding of medicinal plants.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第5期657-660,共4页
China Journal of Chinese Materia Medica
基金
江苏省自然科学基金项目(BK2011815)
关键词
中药
有效成分
半抗原
抗体
人工抗原
traditional Chinese medicine
effective component
hapten
antibody
artificial antigen
