摘要
目的建立了蛇足石杉中石杉碱甲含量的测定方法并比较植物不同药用部位石杉碱甲的含量。方法采用DiamonsilTmC18柱(200mm×4.6mm,5μm),流动相为甲醇∶0.08mol·L-1醋酸铵缓冲液(40∶60,pH=6.0),检测波长为308nm,流速为1.0ml·min-1,柱温为室温。结果石杉碱甲在5～100mg·L-1浓度范围线性关系良好(r=0.9997),平均回收率为98.10%,RSD为2.44%(n=5),对蛇足石杉不同药用部位的进行了石杉碱甲含量测定,结果表明,叶中石杉碱甲含量最高,茎次之,根最低。结论本法简便、准确、重复性好。石杉碱甲集中分布在植物的地上部分中。
Aim To establish a HPLC method for determination of the content of Huperzine A in Huperzia serrata and compare it in different parts of the plants. Method Diamonsil^Tm C18 column（200 mm×4. 6 mm,5μm） was used ; the mobile phase was methanol ： 0. 08mol·L^-1 ammonium acetate buffer （ 40 ： 60, pH =6.0） ,the detection wavelength was 308 nm, with flow rate at 1.0 ml·min^-1 ; the column temperature was room temperature. Results There is a good linear relationship within therange of 5 ～100 mg ·L^-1 （ r = 0. 9997 ）. The average recovery was 99. 10% ,with RSD 2.44% （n = 5 ）, Regarding the content of Huperzine A in different parts of Huperzia serrata, it was found that the quantity of Huperzine A in leaf was higher than that in stem and it was the lowest in root. Conclusion The method is simple, accurate and reliable etition. Huperzine A can be accumulated in the aboveground part of the plant.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第8期1017-1018,共2页
Chinese Pharmacological Bulletin
