摘要
目的建立小菜蛾抗菌肽CA的高效液相色谱测定方法。方法色谱柱为Agilent Alltima C18(4.6 mm×250 mm,5μm),流动相为甲醇-乙腈-0.05%三氟乙酸溶液(2.5∶2.5∶95),检测波长为275 nm,柱温为25℃,流速为1.0 mL·min-1。结果小菜蛾抗菌肽CA的线性范围为0.08～0.800μg(r=0.999 4,n=6),平均回收率分别为99.69%,RSD为1.20%(n=6)。结论本法快速、简便、准确,重现性好,可用于小菜蛾抗菌肽CA的定量分析和质量控制。
OBJECTIVE To establish a method for determination of cecropin from PluteUa xylostella （ pxCECA1 ） by RP-HPLC. METHODS An Agilent Alltima C18 column （4. 6 mm ×250 mm,5 μm） was adopted, and the mobile phase was composed of methanol-acetonitrile-0. 05 % TFA （2. 5 ： 2. 5 ： 95 ） at a flow rate of 1.0 mL ·min^-1. The detection wavelength was set at 275 nm and the column temperature was 25 ℃. RESULTS The linear range of the calibration curve for cecropin was 0. 08 - 0. 800 μg （ r = 0. 999 4, n -- 6）. The average recovery was 99. 69% , and RSD was 1.20% （ n = 6） , respectively. CONCLUSION The method is rapid, simple, and accurate with good reproducibility. It can be used for quantitative analysis and quality control of cecropin from PluteUa xylostella.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2013年第6期470-472,共3页
Chinese Pharmaceutical Journal
基金
国家“重大新药创制”科技重大专项资助项目(2012ZX09J12108-04C)
