摘要
目的:观察水飞蓟宾对人肝星状细胞LX-2凋亡与α1(I)胶原mRNA表达的影响。方法:人肝星状细胞株LX-2分为对照组与实验组,对照组以体积分数10%胎牛血清处理,实验组分别以体积分数10%胎牛血清和终浓度分别为6.25mg/L、12.5mg/L、25mg/L、50mg/L的水飞蓟宾处理48h后,应用流式细胞仪Annexin/PI双染方法检测细胞凋亡,应用RT-PCR检测各组细胞α1(I)胶原mRNA的表达。结果:12.5mg/L、25mg/L、50mg/L水飞蓟宾可促进LX-2细胞凋亡,细胞凋亡率随水飞蓟宾浓度增加而升高(P<0.05);不同浓度的水飞蓟宾明显降低LX-2细胞α1(I)胶原mRNA的表达(P<0.05)。结论:水飞蓟宾可促进肝星状细胞凋亡,抑制α1(I)胶原mRNA的表达。
Aim ： To investigate the effects of silibinin on apoptosis and α1 （Ⅰ） collagen mRNA expression in cultured human hepatic stellate cells （HSCs） LX-2 in vitro. Methods：HSCs were divided into control group and experiment group. HSCs in the control group were treated with 10% fetal calf serum （FCS）/DMEM and HSCs in the experiment group were treated with 10% FCS/DMEM and silibinin with different concentrations （6.25 mg/L, 12.5 mg/L,25 mg/L,50 mg/L） for 48 hours. Flow cytometry was used to evaluate the apoptosis of HSCs using Annexin/PI-staining and RT-PCR was used to determine the etl （1） collagen mRNA expression. Results： Silibinin with different concentrations（ 12.5 - 50 rag/L） could promote HSCs apoptosis and the apoptosis rate raised with the concentration of silibinin （ P 〈 0.05 ）. Silibinin could significantly decrease the α1 （Ⅰ）collagen mRNA expression （P 〈 0. 05 ）. Conclusion： Silibinin can promote apoptosis and inhibit the α1 （Ⅰ） collagen mRNA expression of HSCs.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2009年第3期597-599,共3页
Journal of Zhengzhou University(Medical Sciences)
