摘要
目的:观察芸香苷对常见致病菌体外抑制效果,为实际应用研究提供依据。方法:采用碱溶酸沉法从槐树皮中提取芸香苷并进一步纯化,采用微量稀释法对精制芸香苷进行实验室抑菌试验,并检测其皮肤刺激性与皮肤抗菌效果。结果:精制芸香苷有效成分含量95.2%,其溶液对大肠杆菌和福氏志贺菌MIC值为0.5 g/L,对奇异变形杆菌MIC值为1.0 g/L,对金黄色葡萄球菌和表皮葡萄球菌的MIC值为0.25 g/L。2.0 g/L精制芸香苷溶液对大鼠皮肤无刺激性,涂擦人体前臂皮肤作用2 min,可使皮肤上自然菌由处理前的244 cfu/cm2降低到16 cfu/cm2。结论:芸香苷对细菌繁殖体有明显抑菌作用,对革兰阳性菌抑制作用强于革兰阴性菌。
Objective ： To study the antibacterial effects of rutin on pathogenic strains and provide theoretical data for application. Methods ： Rutin was extracted by alkali - solution and acid - isolation from the barks of Sophora ja- ponica Linn, then further purified. The broth microdilution was used to measure the minimal inhibitory concentration （MIC） for the tested strains. The skin simulation and antibacterial effects on the natural bacteria of skin were ob- served. Results： The purified extract contained 95.2% of rutin. The MICs of rutin solution for Escherichia coli, Shigella.flexneri, Protetas mirabilis, Staphylococcus epidermidis and Staphylococcus aureus were 0.5 g/L, 0.5 g/L, 1.0 g/L, 0.25 g/L, and 0.25 g/L, respectively. 2.0 g/L of rutin showed no sinmlations to the rat skin, and re- duced tile natural bacteria of forearm skin of volunteers from 244 cfu/cm2 to 16 cfu/cm2after smeared for 2 min. Conclusion： Rutin had obvious inhibitory effects on bacterial vegetative form, especially on Gram positive bacteria.
出处
《中国卫生检验杂志》
北大核心
2013年第2期335-337,共3页
Chinese Journal of Health Laboratory Technology
基金
武警后勤学院科研基金面上项目(WYM201010)
关键词
芸香苷
抑菌作用
细菌繁殖体
最低抑菌浓度
Rutin
Antibacterial effects
Bacterial vegetative form
Minimal inhibitory concentration
