摘要
目的:寻找微波辅助提取甘草黄酮的最佳条件,并对微波辅助提取法(MAE)和超声波提取法(UE)进行对比研究。方法:采用MAE和UE提取甘草黄酮,并用4因素、3水平的正交设计优化微波提取溪黄草总黄酮的实验条件,以分光光度法测定提取液中的总黄酮含量。结果:通过实验发现MAE提取总黄酮的最佳条件为:固液比1∶30,乙醇浓度为65%,加热功率为360 W,加热时间为30 min时,MAE(96.11%)明显优于UE(90.47%)。结论:MAE提取率高,且提取时间大大缩短,是一种适合溪黄草总黄酮的提取法。
Objective： To explore the best condition of microwave-assisted extraction（MAE） for the flavonoids from Rab- dosia Serra and to compare with traditional ultrasonic extractio （UE）. Methods： MAE and UE were adopted to optimize the laboratory conditions of flavonoids extraction by the orthogonal design according to 4 factors and 3 standard,and the flavonoids in Rabdosia Serra was determined with spectrophotometry. Results： Appropriate MAE conditions included liquid/solid ratios of 1：30, ethanol concentration of 65%, the power of microwave oven of 360W, and extraction time of 30min; the recovery of flavonoids from Rabdosia Serra with MAE （96.11%） was higher than UE （90.47%）. Conclusion： The experiment results demon- strated higher extracting rate and shorter extracting time. It indicated that MAE was more suitable to extraction of the flavonoids.
出处
《中医药导报》
2013年第2期101-102,共2页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
关键词
溪黄草
总黄酮
微波辅助提取
正交试验
提取工艺
Rabdosia Serra
Flavonoids
Microwave-assisted extraction
Orthogonal experiment
Extraction process
