摘要
【目的】观察nodosin对脂多糖(LPS)刺激RAW264.7细胞NO和炎症细胞因子的影响。【方法】采用四甲基偶氮唑盐(MTT)法测定nodosin对体外培养的小鼠巨噬细胞株RAW264.7细胞增殖的影响;采用LPS刺激RAW264.7细胞使细胞分泌细胞因子,以不同浓度(1.25、2.5、5 mmol/L)nodosin干预,Griess法检测培养上清液中NO水平,酶联免疫吸附(ELISA)法检测白细胞介素-6(IL-6)、IL-10、肿瘤坏死因子-α(TNF-α)的含量。【结果】Nodosin在10 mmol/L内对RAW264.7细胞增殖无显著影响;可呈一定剂量依赖关系地抑制LPS刺激RAW264.7细胞后NO、IL-6、TNF-α的表达,促进IL-10的表达。【结论】Nodosin的抗炎作用与其能抑制炎症细胞因子NO、IL-6、TNF-α表达,促进IL-10的表达有关。
Objective To observe the effect of nodosin on nitric oxide （NO） and cytokines release from RAW264.7 cells induced by lipopolysaccharide （LPS）. Methods Methyl thiazolyl tetrazolium （MTT） assay was used to test the influence of nodosin on the proliferation of in-vitro cultured murine macrophage cell line RAW264.7. The secretion of cytokines from RAW264. 7 ceils was stimulated by LPS, and then was intervened with nodosin at the concentrations of 1.25, 2.5 and 5.0 mmol/L. After intervention, the level of NO in the culture supernatant was tested by Griess Reagent, and the levels of interleukin 6 （IL-6）, IL-10, tumor necrosis factor alpha （TNF-α） were determined by enzyme-linked immunosorbent assay （ELISA）. Results Nodosin had no effect on proliferation of RAW264. 7 cells within 10 mmol/L, but showed dose-dependent effect on inhibiting the release of NO, IL-6 and TNF-α, and on increasing the production of IL-10 in the LPS-stimulated RAW264.7 cells. Conclusion The anti-inflammatory action of nodosin is probably related with the inhibition of inflammatory factors of NO, IL-6 and TNF-α, and with the increase of IL-10 expression.
出处
《广州中医药大学学报》
CAS
北大核心
2013年第2期211-213,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
