摘要
目的研究卵黄高磷蛋白对体外培养的人牙髓细胞增殖的影响。方法 4个实验组分别采用1、10、100、1000ng/mL的卵黄高磷蛋白,对照组采用10%胎牛血清,分别作用于体外培养的第6代人牙髓细胞,每组8个标本,培养3、6day后分别对每组作MTT法检测,OD值进行t检验。实验组用100ng/mL卵黄高磷蛋白,对照组用10%胎牛血清作用上述人牙髓细胞,培养48h,常规消化,固定,经DNA荧光染色后,用流式细胞仪测定DNA含量。结果 1～100ng/mL卵黄高磷蛋白作用3、6day均促进人牙髓细胞增殖。10、100ng/mL卵黄高磷蛋白作用6day,对人牙髓细胞增殖的促进作用与对照组相比有显著差异(P<0.05)。1000ng/mL卵黄高磷蛋白作用3、6day均抑制人牙髓细胞增殖。100ng/mL卵黄高磷蛋白作用人牙髓细胞,与对照组相比DNA合成前期细胞比例明显降低,而DNA合成期细胞比例和细胞增殖指数均显著增高。结论卵黄高磷蛋白具有促进人牙髓细胞增殖和DNA合成的作用,可能主要通过促进处于合成前期的细胞进入合成期来实现的。
Objective To investigate the effect of phosvitin on human dental pulp cell （HDPCs） proliferation. Methods The growth of HDPCs treated with phosvitin at different concentrations （ 1, 10 , 100,1000ng/mL） and the 10% bovine serum both for 3d and 6d was examined by MTY assay. The cell cycle in HDPCs treated with 100ng/ mL phosvitin for 48h was observed by flow cytometry. Results Cell growth was enhanced in groups treated with 10 and 100 ng/mL phosvitin for 6d, which was significantly different with the control（P 〈 0. 05）. The proportion of HDPCs treated with 100ng/mL phosvitin in G1 phase of cell cycle was reduced compared with the control. The proportion in S phase and cell proliferation index Prl （ （ S＋ G2M） % ） of HDPCs were significantly higher than that of control. Conclusion Phosvitin enhanced HDPCs growth and DNA synthesis, which may be achieved by activating cells in G1 phase to S phase of cell cycle.
出处
《北京口腔医学》
CAS
2013年第1期27-29,共3页
Beijing Journal of Stomatology
