摘要
目的 :检测α -平滑肌肌动蛋白 (α-SMA)在正常及诱导分化的人牙髓细胞中的表达变化 ,探讨α -SMA与人牙髓细胞分化之间的关系。方法 :体外连续人牙髓细胞 ,传代后分二组 ,一组常规培养 ,一组用条件培养液诱导其分化 ,采用免疫组化和ELISA方法检测两组细胞α -SMA的表达 ,分析其差异。结果 :随体外常规连续培养 ,人牙髓细胞α -SMA表达逐渐增多 ;较之常规培养组 ,诱导分化组的细胞α -SMA表达减少。结论 :人牙髓细胞在朝成牙本质细胞方向分化时 ,α -SMA表达逐渐减少 ;人牙髓细胞体外常规连续培养 。
Objective:To detect the expression of α-SMA in normal and differentiated human pulp cells and to investigate the relationship between α-SMA and the differentiation of human pulp cells.Methods:Passaged human pulp cells were divided into two groups.One was cultured conventionally; the other one was stimulated to differentiation.α-SMA of both groups were detected by immunohistochemistry and ELASA.Results:With commonly continued culture,the expression ofα-SMA was increased.Compared with conventional group,the expression of α-SMA was decreased in cells stimulated.Conclusions:During the differentiation of human pulp cells to odontoblasts, the expression ofα-SMA was declined; with commonly continued culture in vitro, human pulp cells differentiated to mature fibroblasts.
出处
《口腔医学研究》
CAS
CSCD
2003年第3期187-189,共3页
Journal of Oral Science Research
基金
湖北省科委基金支助 (编号 :30 4130 5 78)
