摘要
安卓奎诺尔(Antroquinonol)具有很好的抗癌效果。为了提高樟芝固态发酵中Antroquinonol的产量及其萃取效率,为其工业化生产提供理论参考,本文考察了谷物种类、装料量、初始含水量、外加碳源、氮源含量对樟芝固态发酵产Antroquinonl的影响,并进行了多级逆流浸提方法萃取樟芝固态发酵产品中Antroquinonol的实验。结果表明:优化后的条件为:在1L三角瓶中,大米装料量为110g,初始含水量为50%,外加碳源为2.0g葡萄糖,氮源添加量为0.3g大豆粉。固态发酵中Antroquinonol产量提高了14.44%;用体积分数为95%的乙醇作萃取剂(乙醇与萃取物料比例为10∶1,v/w),以50℃水浴振荡萃取1h为浸提条件进行三级逆流浸提,浸提萃取后残渣中的Antroquinonol残留率小于1%。该法具有溶剂使用量少,萃取效率高的优点。
Antroquinonol, a metabolite produced from solid-state fermentation of Antrodia camphorata has been shown great anti-cancer effect.This study investigated the effects of a number of factors including fermented grain types,substrate capacity, initial moisture content （containing the water of seeds）, supplementary carbon source （ glucose）, nitrogen content on Antroquinonol content by Antrodia cinnamomea and the multi-stage countercurrent extraction method for extracting Antroquinonol from the solid-state fermentation products of Antrodia carnphorata in order to improve the production of Antroquinonol in solid-state fermentation,the extraction efficiency, decrease loss and provide theoretical basis for preparation of Antroquinonol at industry scale.The results were as follows,the best compositions of fermentation medium for Antroquinonol content were as follows, rice 110g, initial moisture content 50% （ with inoculated seed liquid water）, carbon source 2.0g （ glucose）, nitrogen content 0.3g （ soybean powder）.With these optimum conditions,Antroquinonol content increased by ]4.44% than the initial value;After three -stage countercurrent extraction by 95% ethanol （volume ratio of ethanol and fermentation product was 10：1 ,v/w）in 50℃ water bath and shaking for 1h,the results showed that the residual rate of Antroquinonol in the fermentation products was less than 1 %.This method showed the less solvent used and higher extraction efficiency.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第5期164-168,共5页
Science and Technology of Food Industry
关键词
多级逆流萃取
樟芝
固态发酵
优化
Antroquinonol
multistage countercurrent extraction
Antrodia camphorata
solid - state fermentation
optimization Antroquinonol
