摘要
目的 研制一种普通大鼠胰腺癌模型。方法 切开SD大鼠胰腺被膜及部份胰腺实质 ,深 1mm ,置入 9mg的二甲基苯并蒽 (DMBA) ,缝合胰腺被膜。结果 3～ 5个月内大鼠胰腺癌发生率为 6 5 % (33/ 5 0 ) ,其中 30只为胰腺导管上皮腺癌 ,镜下癌细胞呈腺管样分布 ,腺腔大小不一 ,核异型明显 ,并可见癌细胞巢 ;3只大鼠形成胰腺纤维肉瘤 ,胰腺完全消失 ,被弥漫分布的纤维肉瘤细胞取代。结论 采用较大剂量的DMBA直接置入胰腺被膜下的实质内 ,可在短期内获得较高发生率的鼠胰腺癌模型。
Objective To develop a model of pancreatic cancer in common Sprague Dawely rat.Methods The membrane and partial parenchyma (about 1 mm depth) of rat pancreas was opened to put a high dose (9 mg) of dimethylbenzanthracine(DMBA), and then the incision was closed with interrupted sutures. Results The prevalence of pancreatic cancer among DMBA-treated rats within 3-5 months was 65% (33/50). Among the 33 rats with pancreatic cancer, 30 rats developed pancreatic ductal adenocarcinomas with glandular duct-like distribution of cancer cells, various sizes of glandular cavities, obvious nuclei heterotpic and atypical cocus of cancer cells under a microscope. And the remaining 3 cases were diagnosed having pancreatic fibrosarcoma with a great number of fibrosarcoma cells substituting for pancreas.Conclusion High dose of DMBA directly put into the parenchyma of pancreas under the membrane could obtained a pancreatic cancer model with high incidence in a short time.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第5期462-463,I003,共3页
Chinese Journal of Experimental Surgery
基金
卫生部科学研究基金!资助项目 (981134)
关键词
胰腺癌
二甲基苯并蒽
SD大鼠
动物模型
Pancreatic cancer
Dimethylb enzanthracine
Sparague Dawely rat
