摘要
目的建立1株大鼠源胰腺癌细胞系R-PC。方法利用胰腺包膜下埋二甲基苯并蒽(DMBA)致癌药物的方法建立大鼠胰腺癌模型,用组织块法和胶原酶消化法进行原代培养,观察细胞形态学特征,研究细胞生长状态,测定细胞周期,计数染色体数目,组织化学染色和异种移植。结果经过11个月的体外培养,连续传代80余次；细胞生长迅速,倍增时间为34 h；染色体数目在42～76之间。免疫组织化学染色显示细胞角蛋白、细胞角蛋白19和细胞角蛋白20阳性；异种移植成瘤率为100%。结论R-PC是高度恶性的大鼠胰腺癌细胞系,为体内和体外研究恶性细胞的增值和发展提供了优秀工具。
Objective To establish a rat pancreatic cancer cell line R-PC. Methods A new pancreatic cancer cell line was established from pancreatic cancer in the rat induced by DMBA. The cells were cultured by explant culture and enzymatic digestion. Serially passaged cells were characterized by morphology, cytogenetics, immunocytochemistry analysis and xenografts into nude mouse. Results The new pancreatic carcinoma cell line was established from rat pancreatic adenocarcinoma. The cell line has been maintained for over 80 passages during 11 months. Cells grew rapidly, with a doubling time of 34 h. Chromosome number ranged from 42 to 76. The histochemical staining of keratin, cytokeratin 19 and cytokeratin 20 were all positive, and the ratio of heterotransplantation was 100%. Conclusion R-PC was demonstrated as a high malignant rat pancreatic cancer cell line and can be a useful model to study the malignant cell proliferation and differentiation in both in vivo and in vitro.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第6期692-694,共3页
Chinese Journal of Experimental Surgery
关键词
胰腺癌
细胞培养
生物学
Pancreatic carcinoma
Cell culture
Biology
