摘要
去蛋白灵芝多糖是从芝灵Ganoderma lucidum(Leyss·ex Fr.)Karst子实体中提取的纯多糖部分,根据分子量不同,命名为GL-A,GL-B和GL-C。混合淋巴细胞培养反应是同种异型抗原(主要组织相容性抗原)刺激的特异性免疫反应。GL-A(16～250μg·ml^(-1)),GL～B(62～250μg·ml^(-1)),GL-C(16～250μg·ml^(-1))都可以明显促进混合淋巴细胞培养中脾细胞对[~3H]TdR的摄取。而且促进作用与药物浓度之间呈正相关。三种灵芝多糖还可以在同样条件下增加脾细胞中DNA多聚酶α的活性。但是在无细胞体系中,同样的药物浓度反而抑制[~3H]TTP参入酸不溶物。以上结果提示,去蛋白灵芝多糖可以促进由同种异型抗原刺激的淋巴细胞转化作用。其作用机制是通过间接诱导DNA多聚酶的产生,从而促进免疫细胞中DNA的合成和促进细胞的增殖,加速免疫应答过程。
Ganoderma polysaccharides, GL-A, GL-B and GL-C, are isolated from Gano-derma lucidum (Leyss. ex Fr.) Karst. GL-A (16-250 μg·ml^(-1)), GL-B (62-250 μg·ml^(-1)) andGL-C (16-250 μg·ml^(-1)) markedly increased the rate of [~3H] TdR uptake by spleen cells inmixed lymphocyte culture (MLC) and the effects were positively correlated with concentrationsof the drugs. All of the three polysaccharides significantly potentiated the activity of DNApolymerase α in intact spleen cells and the patterns of the effects were very similar to those inabove experiments. However, in cell-free system, the drugs exerted some inhibitions on the[~3H] TTP incorporation into acid-insoluble materials. The results demonstrate that the Ganod-erma polysaccharides increase the DNA synthesis of spleen cells in MLC by enhancement ofDNA polymerase α induction.
出处
《北京医科大学学报》
CSCD
1991年第4期329-333,共5页
Journal of Peking University(Health Sciences)
基金
Project supported by the Fund of Chinese National Educational Commission,NO.8910
关键词
灵芝多糖
灵芝
DNA多聚酶
脾细胞
Ganoderma lucidum
polysaccharide
DNA polymerase α
Mixed lymphocyte culture
