摘要
植物雌激素属于植物衍生的非甾体化合物,可与雌激素受体结合模拟雌激素的作用,其对人类健康的潜在有益作用已成为目前国内外研究的热点。然而,植物雌激素对生殖系统的副作用研究报道较少,本文利用免疫组化和蛋白免疫印迹技术,比较17β-雌二醇(17β-estradiol,E2)、黄体酮(progesterone,P4)以及植物雌激素染料木素(genistein,Gen)、白藜芦醇(resveratrol,Res)、根皮素(phloretin,Phl)对去卵巢大鼠子宫内膜血管渗出和血管通透性的影响。以假手术大鼠为正常对照,每天分别给去势鼠皮下注射E2、E2合并P4、P4、Gen、Res或Phl,连续21d。大鼠子宫组织HE染色显示,去势鼠皮下给予E2、E2和P4联合处理均可诱发嗜酸性粒细胞的渗出;免疫组化和Western blot分析显示,这些去势鼠同时伴有子宫内膜VEGF、NF-κB和TNF-α表达明显上调;而Gen、Res、Phl三种植物雌激素处理后未发现子宫内膜嗜酸性粒细胞渗出及VEGF、NF-κB和TNF-α表达明显改变。结果表明:E2单独使用或E2和P4联用可通过上调VEGF、TNF-α和NF-κB的表达提高子宫内膜血管通透性,诱发去势鼠子宫内膜嗜酸性粒细胞的渗出,而同剂量的植物雌激素Gen、Res和Phl则无明显作用。
Phytoestrogens, a group of plant-derived non-steroidal compounds that can behave as estrogens by binding to estrogen re- ceptors, have drawn great attention for their potentially beneficial effects on human health. However, there are few studies investigat- ing the potential side effects of phytoestrogens on the reproductive system. The present study was to elucidate the effects of 17β-estradiol （E2）, progesterone （P4）, and phytoestrogens genistein （Gen）, resveratrol （Res）, and phloretin （Phl） on eosinophilic infil- tration of the ovariectomized rat uterus and endometrial vascular permeability, and to analyze the underlying mechanisms. The ova- riectomized rats received daily subcutaneous injections of E2, E2＋P4, P4, Gen, Res, Phl, or an equivalent volume of vehicle for 21 days, and sham-operated animals （Sham rats） were used as the controls. Hematoxylin-eosin staining revealed a marked increase in uterine eosinophilic infiltrations in ovariectomized rats treated with E2, E2＋P4 or P4, which was associated with increased expression of vascular endothelial growth factor （VEGF）, nuclear factor-κB （NF-κB）, and tumor necrosis factor-α （TNF-α） proteins as deter- mined by immunohistochemical and Western blot analysis. However, all three phytoestrogens had no markedly effect on the uterine eosinophilic infiltration and the expressions of VEGF, NF-κB, and TNF-α in the uterus of ovariectomized rats. Our data demonstrate that E2 alone or in combination with P4 increases uterine eosinophilic infiltration which is related with vascular hyperpermeability caused by VEGF, NF-κB and TNF-α, whereas phytoestrogens Gen, Res, and Phl, have no such an effect.
出处
《生理学报》
CAS
CSCD
北大核心
2013年第1期8-18,共11页
Acta Physiologica Sinica
基金
supported by Research Fund from Lanzhou Science and Technology Bureau,China(No.2010-1-88)
the Natural Science Foundation of Gansu Province,China(No.1010RJZA111)
Research Fund from Administration of Traditional Chinese Medicine and Chinese Herb,Gansu Province,China(No.GZK-2010-38)
