摘要
提出了一种合成蛋白质分子印迹聚合物的新方法——待定模板印迹。即不使用任何标准蛋白质,在一个实际样品(鸡蛋清)的存在下合成了印迹聚合物。利用原位聚合法得到印迹整体柱及一根对比柱。比较鸡蛋清在两根柱子中的电泳分离,发现印迹聚合物整体柱能够保留高丰度组分。并观察到部分高丰度蛋白质的谱图信号降低或消失,同时出现新的谱峰,表明相同样品中的低丰度组分得到富集。这预示着研究分子印迹的新途径,即基于其内在复杂性(通过一系列样品本身的印迹聚合物)来分析复杂样品。
A new method, termed as ＂pending templates imprinting＂, to prepare protein-imprinted polymers was proposed. The molecular imprinted polymers were prepared in the presence of a real sample（ chicken egg white） , rather than any known purified proteins. An egg white protein-imprin- ted monolithic column and a control column were prepared by in situ polymerization. By comparing the electrophoretic separations of the sample in the imprinted polymeric and the control capillary, it is found that such a sample-imprinted polymer could retain abundant components of the original sample, the egg white. As a result, the signals of abundant proteins in the sample were removed or reduced in the electropherogram. Meanwhile, new peaks appeared, which stands for the enrichment of the other minor proteins in the same sample. This suggests a new way to investigate molecular imprinting, which means analyzing complicated samples based on their instinctive complexities, i.e. , through a series of polymers just imprinted by themselves.
出处
《分析测试学报》
CAS
CSCD
北大核心
2013年第2期249-252,共4页
Journal of Instrumental Analysis
基金
江苏高校优势学科建设工程
国家自然科学基金资助项目(21275124/B5)
关键词
分子印迹聚合物
待定模板
蛋白质
分离
molecular imprinted polymer
pending templates
protein
separation
