摘要
目的:探讨Raf激酶抑制蛋白(Raf kinase inhibitor protein,RKIP)和磷酸化细胞外信号调节激酶(phosphorylated extracellular signal-regulated kinase,p-ERK)在糖尿病肾病大鼠肾组织中的表达及意义。方法:40只Sprague-Dawley(SD)大鼠随机分为2组:正常对照组(N组)和糖尿病肾病组(M组)。苏木精-伊红(hematoxy-lin-eosin,HE)和过碘酸-雪夫(periodic acid-Schiff,PAS)染色观察肾组织病理改变,免疫组化方法检测各组肾组织RKIP和p-ERK1/2表达,Western blotting检测各组RKIP和p-ERK1/2的表达。结果:M组大鼠随着病程的延长,血糖升高,出现蛋白尿,肾重增加,肾组织内p-ERK1/2明显增高,RKIP降低,与N组相比有显著差异(P<0.05)。结论:RKIP表达的减少及p-ERK的表达增加可能促进糖尿病肾病的进展。
AIM ： To investigate the expression of Raf kinase inhibitor protein （RKIP） and phosphorylated ex- tracellular signal-regulated kinase （p-ERK） in the renal tissues of diabetic rats. METHODS： Forty SD rats were randomly divided into normal control group and diabetic nephropathy group. The pathological changes of the renal tissues were ob- served under microscope with HE and periodicacid-Schiff （PAS） staining. The expression of RKIP and p-ERK1/2 was de- tected by the methods of immunohistochemistry and Western blotting. RESULTS ： Following the extension of duration, the blood sugar of the rats in diabetic nephropathy group increased, with the appearance of proteinuria, increase in kidney weight, increase in renal p-ERK1/2 expression and decrease in RKIP expression as compared with control group. CON= CLUSION： The development of diabetic nephropathy may be accelerated by the decrease in RKIP and the increase in p-ERK.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2013年第2期358-360,370,共4页
Chinese Journal of Pathophysiology
基金
贵州省省长基金黔省专和字[No.(2010)80号]
关键词
糖尿病肾病
大鼠
RAF激酶抑制蛋白
细胞外信号调节激酶
Diabetic nephropathies
Rats
Raf kinase inhibitor protein
Extracellular signal^regulated kinase
