摘要
目的通过定量测定月矾中空栓(儿茶、苦参、青黛、血竭、蛇床子等)中多指标成分,综合评价月矾中空栓的质量。方法采用HPLC法分别测定月矾中空栓中儿茶素、表儿茶素、氧化苦参碱、苦参碱、靛蓝、靛玉红、血竭高氯酸盐,运用综合评分法控制月矾中空栓的质量。结果月矾中空栓中7个成分儿茶素、表儿茶素、氧化苦参碱、苦参碱、靛蓝、靛玉红、血竭高氯酸盐进样量分别在0.115 8～1.158μg(r=0.999 3),0.113 0～1.130μg(r=0.999 1),0.169 0～1.690μg(r=0.999 3),0.269 8～2.698μg(r=0.999 8),0.156 2～1.562μg(r=0.999 7),0.051 80～0.518 0μg(r=0.999 8),0.076 88～0.768 8μg(r=0.999 3)范围内呈良好线性关系。平均回收率(n=5)分别为100.9%,99.9%,100.5%,100.8%,100.9%,99.8%,98.2%。结论本方法能够比较全面的控制月矾中空栓的质量。
AIM To evaluate the quality control of Yuefan Hollow Type Suppository （ Catechu, Sophorae flavescentis Radix, Indigo naturalis, Draconis Sanguis, Cnidii Fructus, etc. ） through the determination of multi-index components in the preparation. METHODS The contents of （＋）-catechin, （-）-epicatechin, matrine, oxymatrine, indigo, indirubin and dracorhodin perchlorate in the preparation were determined by HPLC, respectively. The preparation quality was evaluated by comprehensive score method. RESLUTS The calibration curves of （＋）-catechin, （-）-epicatechin, matrine, oxymatrine, indigo, indirubin and dracorhodin＇perchlorate were line- ar in the ranges of 0. 115 8 -1. 158μg （r =0. 999 3）, 0. 113 0 -1. 130 μg （r =0. 999 1）, 0. 169 0- 1. 690 μg （r=0.9993）, 0. 2698 -2.698 μg （r =0.9998）, 0. 1562 - 1.562 μg （r =0.9997）, 0. 051 80 - 0. 518 0μg （ r = 0. 999 8 ） and 0. 076 88 - 0. 768 8 μg （ r = 0. 999 3 ）, respectively. Their average recoveries （n = 5） were 100.9% , 99.9% , 100. 5% , 100. 8% , 100. 9% , 99.8% and 98.2% , respectively. CONCLUSION This method can be used to the quality control of this preparation.
出处
《中成药》
CAS
CSCD
北大核心
2013年第2期296-301,共6页
Chinese Traditional Patent Medicine
基金
辽宁省科技计划项目
月矾中空栓的药效成分基础与药代动力学研究(2009ZZ5010-26)
