摘要
为观察中草药提取物的体外抑菌效果,按常规法制备中草药复方A(黄芩、连翘、大黄、蒲公英)和复方B(白头翁、黄连、黄柏、秦皮)的水提取物、醇提取物,利用管碟法和试管2倍稀释法测定中草药提取物抑菌圈的大小和对大肠杆菌的最低抑菌浓度(MIC)。结果表明,2种中草药复方对大肠杆菌均有一定的抑制作用,并且醇提物的抑菌效果均优于其水煮提取物(P<0.05)。其中复方A的醇提取物和水煮提取物的MIC分别是15.625mg/mL和31.25mg/mL;复方B的醇提取物和水煮提取物的MIC分别是31.25mg/mL和62.5mg/mL。
Water-and ethonal-extracts from two kinds of Chinese herbal medicine compound A（Scutellaria,Forsythia,Rhubarb,Dandelion） and B（Anemone,Berberine,Cortex phellodendri,Cortex fraxini） were obtained using normal methods and their in vitro antibacterial effects on Escherichia coli and the minimum inhibitory concentration（MIC） were determined by the method of agar cup-plate and two-fold tube dilution.The results showed that all the two kinds of Chinese herbal preparation extracts have dramatically antibacterial effects on E.coli and the ethanol extracts on the antibacterial activity were better than theirs boiling water extracts（P0.05）.In compound A,the MIC of the alcohol and boiled extract was 15.625 mg/mL and 31.25 mg/mL,respectively.In compound B,the MIC of the alcohol and boiled extracts was 31.25 mg/mL and 62.5mg/mL,respectively.
出处
《河南农业科学》
CSCD
北大核心
2012年第6期158-160,共3页
Journal of Henan Agricultural Sciences
基金
周口师范学院青年科研基金资助项目(zknuqn201101)
关键词
中草药
提取物
最小抑菌浓度
Chinese herbal medicine
extraction
minimum inhibitory concentration（MIC）
