摘要
研究D101大孔树脂分离纯化魔芋生物碱工艺条件,确定魔芋生物碱的最佳纯化工艺条件。用90%的乙醇70℃下热回流提取魔芋飞粉中的生物碱,再利用D101大孔吸附树脂进行分离纯化。D101大孔吸附树脂分离纯化魔芋飞粉生物碱的最佳上样量177.4 mg/mL,上样流速为0.5 BV/h;最佳的杂质洗脱剂为无离子水,最佳的生物碱洗脱剂为90%的乙醇。D101大孔吸附树脂对魔芋生物碱进行分离纯化,分离纯化效果好,纯化后生物碱纯度高。
The foundation for the further research of alkaloid in konjac was laid throughthe optimal extraction and purification program to determine the best conditions. To extract the alkaloids in konjac with the temperature of 70 ℃ and the ethanol of 90% under thermal circumfluence, and use the D101 macroporous adsorptive resins purification. The best methods of adsorption volume is 1 ： 2 （concentrated refinery liquor volume ： resin volume） the speed is 0.5 BV/ h, the best elution solvent is 10% ethanol and the best elution solvent of the all alkaloids is 90%. To compare the results of the experiments, the best elution technological parameter was confirmed which provide reference for the follow-up experiments and related industrial production.
出处
《食品工业》
北大核心
2013年第2期95-97,共3页
The Food Industry
基金
陕西省教育厅重点试验室项目(2010JS046)
关键词
魔芋
生物碱
提取纯化
Konjac
alkaloids
extraction and purification
