摘要
目的阐明苦参的抑菌作用及机理,为生产实践提供理论依据。方法采用牛津杯法及液体倍比稀释法对鸡大肠杆菌等常见致病细菌进行了敏感性试验;通过电镜观察,SDS-聚丙烯酰胺凝胶电泳对蛋白表达的研究,生长曲线的绘制以及流式细胞仪对菌体细胞周期的分析,对苦参碱粗提物的抑菌作用及机理进行研究。结果苦参对革兰氏阳性菌、革兰氏阴性菌均有明显抑制作用,抑菌谱广;药物是通过抑制鸡大肠杆菌功能蛋白的表达,从而影响菌体的细胞周期,使I期菌数增加,R期菌数下降,最终菌体不能达到正常对数生长期而直接进入衰亡期;菌体形态结构发生明显变化,表现为菌体缢缩变形,中间凹陷呈规则元宝状,细胞质固缩,质壁分离,最终细胞壁破损,内容物泄漏而使菌体死亡。结论苦参碱对鸡大肠杆菌抑菌机理是抑制调控细菌生长、分裂相关蛋白的合成,阻滞其分裂和生长,并且与细胞内的蛋白质结合,细胞质固缩、解体而死亡。
[Objective] An experiment was carried out to study the bacteriostatic action and mechanism of S.flavescens on E.coli. 01c84010. [Method] Cylinder-plate and liquid doubling dilution, drafted curves, electron microscope, SDS-PAGE and flow cytometry, were ued in the study. [ Results ] The result showed that the extracts had obvious bacteriostatic action upon Gram-positive and Gram-negative bacteria and the bacteristatic spectrum was wide. Though restraining the expression of functional protein, the cell cycle was effected and the count of bacteria in phase I increased and that in phase R reduced. Finally, the E.coli 01 C84010 went into decline phase straightly instead of the normal logarithmic phase. Observation under electron microscope revealed that the E.coli 01 C84010, which depressed on center had been constricted into shoe-shaped gold ingots. The cytoplasm was concentrated, the cell was plasmolysed, then the cell wall was damaged, the contents were leaked out and the cell was killed. [ Conclusion ] It is suggested that the bacteriostatic mechanism is that the matrine inhibited the synthesis of proteins associated with growth and cleavage of bacteria, then blocked their division and development. Furthermore, the drug combined with the protein, made the cytoplasm pyknosis and killed the bacteria finally.
出处
《中国农业科学》
CAS
CSCD
北大核心
2006年第5期1018-1024,共7页
Scientia Agricultura Sinica
基金
辽宁省教育厅攻关项目(99096)
关键词
苦参
苦参碱
抑菌作用
抑菌机理
鸡
Sophoraflavescens Ait
Matrine
Bacteriostatic action
Bacteriostatic mechanism
