摘要
研究了中华鲟幼鱼经不同浓度铜(Cu2+)水体(低浓度组(0.40μg/L)、中浓度组(0.89μg/L)、高浓度组(2.00μg/L)、对照组(0.00μg/L))暴露60d后,铜在幼鱼不同组织中的积累以及对幼鱼生长、消化酶活性的影响.结果显示,随着水体Cu2+浓度的增加,幼鱼体质量显著减轻,低浓度组与对照组(无Cu2+水体)无显著性差异(P>0.05),中高浓度组与对照组均有显著性差异(P<0.05).对照组中华鲟幼鱼8种组织中铜含量的顺序从高到低依次为肝脏>鳃>背骨板>肌肉>皮肤>脊索>软骨>消化道;经不同浓度Cu2+水体暴露60d后,随着Cu2+浓度的升高,8种组织中铜含量与对照相比均显著增加(P<0.05),肝脏中铜的含量仍最高;消化道中铜含量增加最多,低浓度组是对照组的21.41倍,高浓度组是对照组的58.58倍;表明肝脏和消化道是Cu2+主要的累积和代谢器官.随着Cu2+浓度升高,消化道蛋白酶、淀粉酶的活性与对照组相比显著下降(P<0.05);脂肪酶活性则随着Cu2+浓度的升高而升高,低浓度组与对照差异不显著,中高浓度组与对照组均有显著性差异(P<0.05).表明Cu2+对蛋白酶和淀粉酶的活性有抑制作用,对脂肪酶活性有促进作用.代谢酶活性的变化可以灵敏地反映Cu2+的胁迫程度和毒性.
Copper accymulation, and its effects on growth and digestive enzyme activities were investigated in juvenile Chinese sturgeon, Acipenser sinensis （mean weight 141. 52 ± 5. 76 g）, after dissolved Cu^2＋ exposure （0. 00, 0. 40, 0. 89, 2. 00 μg/L）for 60 days. Cu^2＋ reduced the weight increasing. There was an inverse relationship between growth and Cu^2＋ concentration. Cu^2＋ accumulation in tissues was dependent on the exposure time and Cu^2＋ concentration. In the control group, Cu^2＋ content was the highest in liver（2. 31 ± 0. 16μg/g dry weight）and lowest in digestive tract（0. 12±0. 02μg/g dry weight）. The order of Cu^2＋ content among tissues was liver gill 〉 dorsalbone plate 〉 muscle 〉 skin 〉 notochord 〉 cartilaginous bone 〉 digestive tract. Cu^2＋ content among tissues was significantly rising with the increasing Cu^2＋ concentration. In test groups, Cu^2＋ content in liver was still the highest. Fish exposed to lower and higher concentration（0. 40, 2. 00 μg/L） for 60 days showed significantly higher copper accumulation to the control group（0. 00 μg/L）in liver, increasing by 21.41, 58. 58 times, respectively. Cu^2＋ content increasing was the largest in digestive tract, which was 21.41, 58. 58 times in lower and higher concentration to the control group, respectively. Cu^2＋ content in cartilaginous bone was the lowest in lower concentration and medium concentration（0. 89 μg/L）. That in higher concentration was higher than notochord and dorsalbone plate. Cu^2＋ inhibited the proteinase and amylase activity and promoted lipase activity. The results showed that liver and digestive tract were more important copper storage and metabolic tissues than other tissues. The changes of digestive enzyme activity can sensitively reflect the degree of Cu^2＋ stress and to：deity.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2012年第6期789-795,共7页
Journal of Fudan University：Natural Science
基金
公益性行业(农业)科研专项资金资助项目(201203065)
上海市长江口中华鲟自然保护区专项资金资助项目(2012)
中央级公益性科研院所基本科研业务费专项资金资助项目(东海水产研究所2011M08)
关键词
中华鲟幼鱼
铜
生长
消化酶
积累
juvenile Chinese sturgeon
copper
growth
digestive, enzyme
accumulation
