摘要
目的研究羟基红花黄色素A(HSYA)对血管内皮细胞黏附功能的影响。方法选用猪髋动脉内皮细胞(PIECs)作为细胞模型,应用同位素氚与胸腺嘧啶结合的方法标记细胞,通过检测放射性计算出在共培养不同时段黏附于聚酯膜上的细胞数,利用Rh 123染色显示黏附细胞中的线粒体活性。结果在HSYA作用24h后,与对照组相比HSYA明显升高黏附细胞数量,并且这一作用呈现出剂量依赖性的现象(P<0.05),同时内皮细胞中的线粒体荧光增强,也呈剂量依赖性(P<0.05),都在HSYA浓度为0.007 3g/L达到最理想效果。结论 HSYA能够促进内皮细胞的黏附和提高内皮细胞线粒体活性。
Objective To evaluate the effect of hydroxy safflor yellow A （HSYA） on endothelial cells function. Methods PIECs were chosen as cell model. PIECs were labeled with isotope through the combination of 3H-thymidine. The radioactivity of cells was detected to determine the number of cells attached to the material surface following the time course. The activity of adhesive cells was measured by rhodamine123 staining. Results HSYA had significant impact on cell adhesion and activity compared with control group after 24h （P〈0.05）, and this showed dose- and time-dependently increased. The action was most powerful when the concen- tration of HSYA was 0. 007 3 g/L. Conclusion HSYA could promote adhesion and increase mitochondrion viability of PIECs.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第5期493-495,共3页
Chongqing medicine
基金
黑龙江省科技厅国际合作资助项目(WB08B06)
黑龙江省博士后基金资助项目(LBH-09002)
黑龙江中医药科研资助项目(ZHY12-Z158)
关键词
内皮细胞
动脉硬化
红花黄色素
endothelial cells
arteriosclerosis
safflor yellow
