摘要
本文探讨了葛根素对牛内皮细胞的作用。在细胞培养液中加入葛根素(250μg/ml)为实验组;不加则为对照组。经37℃6h 培养后,分别测定内皮细胞内外液中羟(?)氨酸和糖胺多糖的含量。结果表明,葛根素对牛内皮细胞有明显作用,羟脯氨酸含量由对照组细胞内液和外液中的3.35±0.05μg/ml 和2.68±0.10μg/ml 下降至实验组的2.70±0.06μg/ml(P<0.01)和2.43±0.21μg/ml(P<0.05);糖胺多糖由对照组细胞内液和外液的1.17±0.12μg/ml 和1.57±0.08μg/ml,降至实验组的0.95±0.12μg/ml(P<0.01)和1.68±0.15/μg/ml。
In order to explore the effects of Puerarin on arterial endothelial cells of calf,endothelial cells were cul- tured with mediums M-199 which contained 0 and 250 μg/ml of Puerarin and compared with the control group.After six hours culturing in 37℃,the concentrations of hydroxyproline and glycosaminoglycan(GAG) in the intracellular and extracellular fluids were measured.The concentrations of hydroxyproline in the extra- cellular and intracellular fluid in control group were 2.68(μg/ml)and 3.35 within the experiment groups 2. 43(P<0.05)and 2.70(P<0.01).The concentrations of GAG in the extracellular and intracellular fluid of the control group were 1.57(μg/ml)and 1.17,with 1.68 and 0.95(P<0.01)in the experiment group.It is concluded that Puerarin has obvious effects on arterial endothelial cells of calves.
出处
《老年学杂志》
CSCD
1992年第6期350-351,共2页
关键词
葛根素
内皮细胞
羟脯氨酸
Puerarin
Endothelial cells
Hydroxyproline
Glycosaminoglycan(GAG)
