摘要
目的检测赖氨酰氧化酶(LOX)在肝癌细胞株中的表达及对肝癌细胞株侵袭性的影响。方法采用实时定量PCR方法检测不同肝癌细胞株中LOX mRNA的表达,Transwell小室侵袭实验观察不同浓度β-氨基丙腈(BAPN)对高侵袭性细胞株SK-Hep-1细胞侵袭性的影响。结果不同细胞株HepG-2、L02、MDA-MB-231、QGY7703、SK-Hep-1、SMMC-7721中LOX mRNA相对表达量依次为3.07、1.00、1.17、0.44、6.49、0.33。LOX mRNA在高侵袭细胞株SK-Hep-1中表达明显高于其他细胞株。加入不同浓度BAPN(0、0.1、0.2、0.3μmol/L,依次为1、2、3、4组)的SK-Hep-1细胞24h后穿膜数分别为(57.33±6.02)、(41.00±3.61)、(19.67±4.04)、(13.33±2.52)个。1组与3组(P<0.05)及与4组(P<0.01)、2组与3组(P<0.05)及与4组(P<0.01)差异均有统计学意义。结论高侵袭性细胞株SK-Hep-1高表达LOX mRNA,降低其LOX活性后侵袭能力明显下降。
Objective To investigate lysyl oxidase（LOX） expression and its effect on the invasion of hepatocelluar carcinoma cell lines. Methods The LOX mRNA expression was quantified in different kinds of hepatocelluar carcinoma cell lines by RT-PCR assay.The influence of different concentrations of β-aminopropionitrile（BAPN） on invasion of SK-Hep-1 cell line was quantified by Transwell cell invasion assay. Results The expressions of LOX mRNA in different cell lines of HepG-2,L02,MDA-MB-231,QGY7703,SK-Hep-1,SMMC-7721 were 3.07,1.00,1.17,0.44, 6.49,0.33,respectively. LOX mRNA expression was higher in SK-Hep-1 than that in the other cell lines.The numbers of transmembraned SK-Hep-1 cells treated with different concentrations of BAPN（0,0.1,0.2, 0.3μmol/L,named groups of 1,2,3,4） were （57.33±6.02）,（41.00±3.61）,（19.67±4.04）,（13.33±2.52）,respectively. Transmembraned cells were more in group 1 than those in group 3（P〈0.05） and group 4（P〈0.01）,also transmembraned cells were more in group 2 than those in group 3（P〈0.01） and group 4（P〈0.05）. Conclusion LOX mRNA is highly expressed in the invasive cell line SK-Hep-1. The ability of cell invasion is decreased by reducing the activity of LOX.
出处
《江苏医药》
CAS
北大核心
2013年第2期138-141,共4页
Jiangsu Medical Journal
关键词
赖氨酰氧化酶
肝癌细胞株
Lysyl oxidase
Hepatocelluar carcinoma cell lines
