摘要
目的探讨不同转移潜能的肝癌细胞株中几种CD标记物的表达差异及其意义,为进一步利用CD分子标志物分离多潜能肝癌干细胞奠定基础。方法常规培养三种不同转移潜能的肝癌细胞株HepG2、MHCC-97H、SK-HEP-1,胰酶消化获得单细胞悬液后,经流式抗体染色,通过流式细胞仪分析三种肝癌细胞株中CD133、CD90、CD44、CD34、CD24的表达差异及意义。结果 CD133+细胞在HepG2细胞株仅占0.1%,而在MHCC-97H、SK-HEP-1细胞中表达也较低;CD90+、CD44+、CD90+CD44+表达水平随细胞转移潜能的增加呈递增趋势(P<0.05)。CD44+CD24+在HepG2、MHCC-97H、SK-HEP-1细胞中的阳性表达率分别为0.05%、10.3%和0.3%(P<0.05);三者中均无CD34+和CD133+CD90+细胞表达。结论 CD90和CD44与肝癌细胞的转移潜能有一定的相关性,可能成为潜在的肝癌肿瘤干细胞标记物。
Objective To evaluate the expression of several CD cell markers and investigate their significance in hepatocellular carcinoma cell lines with different metastatic potential. Methods Three human hepatocellular cell lines,HepG2,MHCC-97H and SK-HEP-1 were cultivated by routine method.Single cell suspension was achieved by digestion with trypsin.After CD133,CD90,CD44, CD34 and CD24 antibody labeling,cells were analyzed by flow cytometry. Results CD133＋ cells represented only a very small subpopulation in three hepatocellular carcinoma cell lines（0.1%~0.4%）.In addition,CD90＋,CD44＋ and CD90＋CD44＋cells were detected in the three cell lines and the percentage increased with the development of metastatic potential（P﹤0.05）.The expression rates of CD44＋CD24＋ cells in HepG2,MHCC-97H and SK-HEP-1 were 0.05%,10.3% and 0.3%,respectively（P﹤0.05）.Furthermore,no CD34＋ or CD133＋CD90＋ expression was observed in the three cell lines. Conclusion CD90 and CD44 are significantly correlated with the metastatic potential of hepatocellular carcinoma cells and may be prospective markers for hepatocellular carcinoma stem cells.
出处
《胃肠病学和肝病学杂志》
CAS
2012年第3期221-223,共3页
Chinese Journal of Gastroenterology and Hepatology
基金
国家自然科学基金资助项目(30873038
30972759)
