摘要
目的:建立参芪养心滴丸中哈巴俄苷和肉桂酸的含量测定方法。方法:采用HPLC法;色谱柱为Kromasil-C_(18)柱(200mm×4.6 mm,5μm);流动相为乙腈-1%醋酸(28:72);流速为1.0 ml·min^(-1);检测波长为278 nm。结果:哈巴俄苷进样浓度在0.68～21.92μg·ml^(-1)范围内呈良好的线性关系,r=0.999 9,平均回收率为98.15%,RSD为1.15%(n=6);肉桂酸进样浓度在1.09～35.00μg·ml^(-1)范围内呈良好的线性关系,r=0.999 9,平均回收率为98.42%,RSD为1.14%(n=6)。结论:该方法无干扰,准确、重复性良好,可作为产品的质量控制方法。
Objective：To establish a method to determine harpagoside and cinnamic acid in Shenqi Yangxin guttate pills.Method： HPLC was used to determine harpagoside and cinnamic acid on a Kromasil-C_（18）（200 mm×4.6 mm,5μm） column using acetonitrile -1%acetic acid（28：72） as the mobile phase.The flow rate was 1.0 ml·min^（-1） and the detection wavelength was at 278 nm.Result： There was a good linearity within the range of 0.68 -21.92μg· ml～（r=0.999 9） for harpagoside,and the average recovery was 98.15%with RSD of 1.15%（n =6）.There was a good linearity within the range of 1.09-35.00μg· ml^（-1）（r =0.999 9） for innamic acid and the average recovery was 98.42%with RSD of 1.14%（n = 6）.Conclusion：The method is simple,accurate and reproducible. It can be used as the quality control method of Shenqi Yangxin guttate pills.
出处
《中国药师》
CAS
2012年第12期1703-1705,共3页
China Pharmacist
基金
2010军队中医药科研专项重点课题(编号:10ZYZ101)
