摘要
目的测定排石颗粒(连钱草、木通、徐长卿等)中5种指标成分和总黄酮的含有量。方法 RP-HPLC法测定排石颗粒50%甲醇提取液中各成分含有量,分析采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×150 mm,5μm);以乙腈-0.05%磷酸为流动相,梯度洗脱;检测波长240 nm;体积流量1.0 m L/min;柱温30℃。紫外分光光度法测定总黄酮的含有量。结果绿原酸、马钱苷、芦丁、迷迭香酸、甘草酸铵分别在18.88~604.00、18.50~592.00、20.25~648.00、18.75~600.00、18.81~602.00μg/m L范围内线性关系良好,平均加样回收率分别为97.9%、100.7%、103.3%、96.4%、102.4%,RSD分别为1.4%、0.4%、2.7%、1.2%、2.3%。20批样品中总黄酮含有量均符合药典要求,而指标成分含有量均有一定差异,其中马钱苷变化最明显,芦丁和甘草酸铵次之。结论该方法简便、准确、可靠,可用于排石颗粒的质量控制。
AIM To determine the contents of five marker components and total flavonoids in Paishi Granules（ a medication to remove stones in the body,containing Glechomae Herba,Akebiae Caulis,Cynanchi Paniculati Radix et Rhizoma,etc.）. METHODS The content determination of various components in 50% methanol extract of Paishi Granules was accomplished by RP-HPLC. The analysis was performed on a 30 ℃ thermostatic Aglient SBC18column（ 250 mm × 4. 6 mm,5 μm）,with the mobile phase comprising of acetonitrile-0. 05% phosphoric acid flowing at 1. 0 m L / min in a gradient elution manner,and the detection wavelength was set at 240 nm. The content of total flavonoids was detected by ultraviolet spectrophotometry. RESULTS Chlorogenic acid,loganin,rutin,rosmarinic acid and ammoninm glycyrrhizinate showed good linear relationships within the ranges of 18. 88-604. 00,18. 50-592. 00,20. 25-648. 00,18. 75-600. 00 and 18. 81-602. 00 μg / m L,whose average recoveries were 97. 9%,100. 7%,103. 3%,96. 4% and 102. 4% with the RSDs of 1. 4%,0. 4%,2. 7%,1. 2% and2. 3%,respectively. The contents of total flavonoids in twenty batches of samples all met pharmacopeia requirements,while those of marker components were found to be different,among which loganin displayed the most significant change,followed by rutin and ammoninm glycyrrhizinate. CONCLUSION This simple,accurate and reliable method can be used for the quality control of Paishi Granules.
出处
《中成药》
CAS
CSCD
北大核心
2017年第1期85-89,共5页
Chinese Traditional Patent Medicine
关键词
排石颗粒
指标成分
总黄酮
RP-HPLC
紫外分光光度
Paishi Granules
marker components
total flavonoids
RP-HPLC
ultraviolet spectrophotometry
