摘要
目的通过实验室研究探讨腹腔镜手术对子宫内膜癌细胞转移潜能的影响。方法分别采用流式细胞技术及实时荧光定量RT-PCR检测20例行腹腔镜手术者(腹腔镜手术组)、20例行开腹手术者(开腹手术组)和20例行腹腔镜下全子宫切除术者(空白对照组)手术前后子宫内膜癌细胞凋亡率及MTA1、nm23-H1基因表达水平的改变,分析不同术式对其影响。结果 (1)腹腔镜手术组子宫内膜癌细胞术前及术后凋亡率分别为(3.55±0.60)%和(25.23±2.16)%,差异有统计学意义(P<0.001);开腹手术组子宫内膜癌细胞术前及术后凋亡率分别为(3.58±0.5)%和(3.60±0.52)%,差异无统计学意义(P>0.001);空白对照组正常子宫内膜细胞术前及术后凋亡率分别为(28.23±1.09)%和(28.18±1.32)%,差异无统计学意义(P>0.001)。(2)腹腔镜手术组术前子宫内膜癌组织MTA1、nm23-H1基因表达量分别为(0.062±0.037)和(0.002±0.001),术后基因表达量分别为(0.012±0.008)和(0.005±0.003),差异有统计学意义(P<0.001);开腹手术组术前子宫内膜癌组织中MTA1、nm23-H1基因表达量分别为(0.061±0.034)和(0.002±0.001),术后基因表达量分别为(0.076±0.051)和(0.002±0.001),差异无统计学意义(P>0.001);空白对照组术前正常子宫内膜组织中MTA1、nm23-H1基因表达量分别为(0.002±0.001)和(0.006±0.003),术后基因表达量分别为(0.003±0.001)和(0.006±0.003),差异无统计学意义(P>0.001)。结论腹腔镜手术并不增加子宫内膜癌细胞转移潜能,反而对子宫内膜癌细胞转移能力有抑制作用,是一种安全的手术方法。
Objective To study the influence of laparoscopic surgery on endometrial carcinoma cell metastatic potential. Methods Pre - and post - operative endometrial cancer cell apoptosis and changes of MTA1, nm23 - H1 gene expression levels were detected by flow eytometry and real - time quantitative RT - PCR in 20 patients undergoing laparoscopic surgery （group A）, 20 having laparotomy （group B） and 20 receiving laparoscopic hysterectomy （group C）. The effects of varying surgical methods on it were analyzed. Results （ 1 ） In group A pre - and post - operative cell apoptosis rates were （ 3.55 ± 0.60）%, （25.23 ±2.16）%, respectively, the difference was significant （P〈0.001）; In group B （3.58 ±0.54）%, （3.60±0.52）%, respectively, the difference was not significant （P〉0.001）; In group C （28.23 ±1.09）%, （28.18 ± 1.32）%, respectively, the difference was not significant （P 〉0. 001）. （2） In group A, before operation MTA1, nm23 - H1 gene expression of endometrium cancer tissues were （0. 062 ±0. 037） , （0. 002 ±0. 001） , respectively, and after operation （0.012 ± 0. 008 ）, （0. 005 ± 0. 003 ）, respectively, the difference was significant （P 〈 0. 001 ） ; In group B, before operation （0. 061 ± 0. 034）, （0. 002 ± 0. 001 ）, respectively, and after operation （0. 076 ± 0. 051 ）, （0. 002 ± 0. 001 ）, respectively, the difference was not significant （P 〉0. 001 ） ; In group C, before operation （0. 002 ±0. 001 ） , （0. 006 ±0, 003） , respectively and after operation （0. 003 ± 0. 001 ）, （0. 006 ± 0. 003 ）, the difference was not significant （ P 〉 0. 001 ）. Conclusion Laparoscopic surgery, not increasing endometrial carcinoma cell metastatic potentiality but inhibiting it, is a safe surgical method.
出处
《中国全科医学》
CAS
CSCD
北大核心
2013年第2期164-166,共3页
Chinese General Practice
基金
海南省自然科学基金项目(琼科30867)
关键词
子宫内膜癌
细胞凋亡率
基因表达
转移
Endometrial carcinoma
Cell apoptosis rate
Gene expression
Metastasis
