摘要
为了研究显性低酚杂交棉苏杂501(F1)、苏杂502(F1)的产量杂种优势、抗病虫特性,测定了其棉酚含量、蛋白质含量、氨基酸含量和脂肪含量,结果显示:2个显性低酚杂交棉均具有明显的中亲优势,其中苏杂502(F1)的籽、皮棉产量均具有正向竞争优势,分别比对照(苏杂6号)增产3.41%、0.11%;苏杂501(F1)的籽、皮棉产量表现一定的负向竞争优势,分别比对照减产1.02%、4.72%。苏杂501(F1)和苏杂502(F1)的黄萎病相对病指分别为25.9、24.7,均表现为耐病;对棉铃虫的平均抗性值分别为2.50、3.42,均达抗级水平。去油后苏杂501(F1)、苏杂502(F1)的棉酚含量分别为30.42 mg/kg、39.34 mg/kg;2个杂交组合F2的棉酚含量分别373.22 mg/kg、367.92mg/kg,均在安全饲用指标范围之内。另外,本试验结果显示,利用SSR分子标记构建了2个杂交棉的指纹图谱,利用一对MH0454引物,既可将显性低酚杂交棉与父本、母本区分开来,也可将显性低酚杂交棉与常规显性低酚棉区分开来,从而为鉴别真假低酚杂交棉提供了依据。
To study the yield heterosis and pest and disease resistance of the dominant low gossypol cotton Suza501 and Suza502,the gossypol,protein,fat and free amino acid contents were determined.The results showed that two hybrids（Suza501 and Suza502） expressed obvious mid-parent heterosis.Suza 502 had positive competitive advantage,the yields of unginned and ginned cotton being 3.41% and 0.11% higher than those of Suza 6,respectively.Suza 501 owned a certain negative competitive advantage,the yields of unginned and ginned cotton being 1.02% and 4.72% lower,respectively.Suza 501 and Suza 502 were resistant to Verticillium wilt,with their relative disease indexes of 25.9 and 24.7,respectively.They were both resistant to bollworm,with the average resistance values of 2.50 and 3.42,respectively.After deoil,the gossypol contents of Suza501 and Suza502 were 30.42 mg/kg and 39.34 mg/kg,respectively,and their F2 generation were 373.22 mg/kg and 367.92 mg/kg,respectively,which were all in safety range for animal feed.SSR fingerprints for two hybrid cotton Suza501 and Suza502 was constructed.Using the primer MH0454,the dominant low gossypol cotton from the parents was differentiated,and the hybrid dominant low gossypol cotton from conventional dominant low gossypol cotton was differentiated as well.
出处
《江苏农业学报》
CSCD
北大核心
2012年第6期1229-1234,共6页
Jiangsu Journal of Agricultural Sciences
基金
转基因生物新品种培育科技重大专项子课题(2011ZX08005-001)
转基因特色专用棉新品种培育项目(2011ZX08005-005)
关键词
显性低酚
杂种优势
棉酚
SSR
dominant low gossypol
heterosis
gossypol
SSR
