摘要
目的 分析并克隆镍转化细胞与对照组细胞中差异表达的基因 ,以期了解镍化合物诱发癌变的分子机制。方法 应用差异显示PCR方法分离对照和转化细胞间具有差异表达的基因 ,用TACloning方法将差异基因片段克隆到质粒中 ,循环测序 ,Northern杂交分析。结果 从转化与对照细胞中分离并克隆到多个差异基因片段 ,Northern分析表明其中 2个为确定的差异表达基因 (暂称之为MS 5 15和IC 82 )。测序后与基因库比较表明 ,MS 5 15与已知基因有 80 %以上的同源性 ,而IC 82为全新基因。具体功能有待进一步分析。结论 镍化合物的致癌作用具有多样性 ,不同的镍化合物对不同的细胞其致癌机制不同 ,体现在对不同基因的作用不同。
Objective To analyze and clone the differentially expressed genes in nickel transformed cells or their normal cells. Methods Differential Display PCR(DD PCR),TA cloning method,cycle sequencing and Northern blot analysis were used. Results There were some differentially expressed genes that were isolated and cloned in nickel transformed cells or their normal.Of them, at least two genes(named MS 515 and IC 82) were differential.Compared with Genbank, MS 515 was found to share high homology with reported genes,while IC 82 was completely novel. Conclusions Nickel carcinogenisis is various from nickel compounds and individual cell lines.Different nickel compounds can affect different genes in different cells.MS 515 and IC 82,which were cloned and differentially expressed,might play important roles in nickel induced transformation and carcinogenesis.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2000年第3期161-164,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家教委博士点基金!资助项目 (972 2 )
