摘要
目的研究双黄连口服液和庆大霉素联用对金黄色葡萄球菌、铜绿假单胞菌的体外抗菌作用。方法采用微量溶液稀释法,测定双黄连口服液和庆大霉素分别对金黄色葡萄球菌、铜绿假单胞菌的最低抑菌浓度(MIC);采用棋盘联合药敏试验法,测定双黄连口服液和庆大霉素联用分别对金黄色葡萄球菌、铜绿假单胞菌的部分抑菌浓度(FIC)指数。结果双黄连口服液对金黄色葡萄球菌和铜绿假单胞菌的MIC分别是46.88 mg.mL-1(稀释度1∶32)和375 mg.mL-1(稀释度为1∶4);庆大霉素对金黄色葡萄球菌和铜绿假单胞菌的MIC分别是0.25和1 U.mL-1;双黄连口服液和庆大霉素联用对金黄色葡萄球菌和铜绿假单胞菌的FIC指数均是0.56。结论双黄连口服液联用庆大霉素对金黄色葡萄球菌和铜绿假单胞菌药敏结果均呈相加作用。
Objective To explore the antibacterial effect of shuanghuanglian oral liquid combining with gentamicin in vitro against staphylococcus aureus and Pseudomonas aeruginosa . Methods. The MIC of shuanghuanglian oral liquid or gentamicin against Staphylococcus aureus or Pseudomonas aeru ginosa was detected by a microamount dilution method. The antibacterial activity of shuanghuanglian oral liquid combining with gentamicin was determined by a chessboard dilution method and was assessed according to FIC index. Results These MIC values of shuanghuanglian oral liquid against Staphylococcus aureus and Pseudomonas aeruginosa were 46, 88 mg . mL^-1 and 375 mg . mL^-1, respectively, those of gentamicin against Staphylococcus aureusps and pseudomonas aeruginosa were 0.25 U . mL^-1 and 1 U. mL^-1 , respectively. The FIC indexes of shuanghuanglian oral liquid combining with gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa were all 0.56. Conclusion The antibacterial effect of shuanghuanglian oral liquid combining with gentamicin against Staphylococcus aureus or Pseudomonas aeruginosa is added via an antimicrobial susceptibilities test.
出处
《医药导报》
CAS
北大核心
2013年第1期19-22,共4页
Herald of Medicine
基金
校级自然科学研究课题(KYZ2008206)
