摘要
研究了用水浸提法抽提大青叶中抗菌物质的方法,并通过抑菌实验检验了它对细菌和霉菌的抑菌能力。正交实验结果表明最佳抽提工艺为:料水比1:20,加热时间40min,回流温度100℃;滤纸片法抑菌实验发现:大青叶提抽浓缩液(浓缩比为5:1)对大肠杆菌及枯草杆菌的最低抑菌浓度为2.77%(ml/100ml)左右,青霉,黄曲霉为0.55%(ml/100ml)左右,金黄色葡萄球为0.055%(ml/100ml)左右。
By using water a kind of antiseptic from Cloyodendron Cvrtophyuan which could be added into feed was extracted.By means of filter paper disks tests the antimicrobial effect of Cloyodendron Cvrtophyuan’s extract were verified and then.The extract’s antimicrobial efficeney on Staphylocooous and Penieillium was taken as the measurements in this experiment. After orthogonal design and experiments, the optimal extract process gualifications are extractive ratio is 1:20, circumfluencetemperture is 100℃, circumfluence time is 40min, The marium absorbing peak is 270nm. Cloyodendron Cvrtophyuan haveobvious antimicrobial actions on bacteria and mod . The lowest antimicrobial level for Staphylococcus aureus is 0.055%(ml/100m), Aspergillus flavus and Penicillium is 0.55%(ml/100m),Escherichia coliform and Bacillus subtilis is 2.77%(ml/100m).
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第11期138-140,共3页
Food Science
基金
湖南省教育厅科技基金支助项目(01C008)
