摘要
本文研究建立了一种检测盐酸克伦特罗与莱克多巴胺联检速测卡的方法。应用胶体金免疫层析技术,采用柠檬酸钠还原法制备胶体金颗粒,标记盐酸克伦特罗与莱克多巴胺单克隆抗体并喷于玻璃纤维上,盐酸克伦特罗与莱克多巴胺偶联OVA抗原和羊抗鼠IgG分别结合于硝酸纤维膜上,依次将样品垫、胶体金垫、硝酸纤维素膜和吸水纸组装切割成盐酸克伦特罗与莱克多巴胺联检速测卡。试验结果表明,该联检速测卡的灵敏度分别为CLEN 3 ng/mL、RAC 5 ng/mL,检测时间为3 min,批内和批间重复性为100%,假阳性率和假阴性率均为0。
This study established a method of synchronizating rapid detection with clenbuterol and ractopamine by using colloidal gold immunochromatography and sodium citrate reduction for preparation of colloidal gold particles. To mark clenbuterol and ractopamine monoclonal antibody, and sprayed on glass fiber, the antigen of clenbuterol and ractopamine coupled OVA and sheep anti-mouse IgG were combined with nitrocellulose membrane respectively. Then, the sample pad, colloidal gold pad, nitrocellulose membrane and absorbent paper were assembled and cut into Articles. The results showed that sensitivity of the rapid detection article was 3 ng/mL for CLEN and 5 ng/mL for RAC .The detection time was 3 min and intra-and inter-reproducibility was 100%. False positive rate and false negative rates were 0.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第1期197-200,共4页
Modern Food Science and Technology
